| Objective:To observe the clinical efficacy of Busuishengxue granule on chronic aplastic anemia (CAA) patients. To explore the effect of Busuishengxue granule on CAA's bone marrow hematopoieticadhesion signal transduction Src/PLC/IP3pathway and Ca2+pathway cytokines.Methods:121cases of CAA patients were divided into two groups, experimental group of60cases, the Busuishengxue granule therapy; control group of61cases, treated by Zaizaoshegnxue tablets.3months for a course of treatment, two groups of patients2courses after the clinical efficacy. Bone marrow mononuclear cells fromrelated enzymes Src, the PLC, IP3and its phosphorylation of phospho-Src, phospho-PLC, phospho-IP3of protein expression by Western blot before and after the detectionof CAA patients. Flow cytometry of bone marrow mononuclear cells of intracellular Ca2level changes in CAA patients before and after treatment study. Results:1. Busuishengxue granule group more effective than Zaizaoshengxue tablets group (P<0.05);CAA kidney yang deficiency group more effective than kidney ying deficiency group (P<0.05);2. CAA in patients with bone marrow mononuclear cells Src, PLC, IP3protein and its phosphorylation expression of phospho-Src, phospho-PLC, phospho-IP3of higher than the normal group (P<0.05);3. Busuishengxue granules can be lowered more than several abnormally high protein expression (P<0.05); and treatment after kidney yang and kidney yin between the two has a significant difference (P<0.05).4.CAA patients' BMNC of Ca2+expression level was significantly increased (P<0.05); Ca2+expression levels after Busuishengxue granule treatment lower (P<0.05).Conclusion:1.CAA fundamental pathogenesis of kidney marrow dificiency, the exact effect of Busuishengxue granules treatment, suggesting that the treatment of CAA from the kidney.2. Bone marrow mononuclear cells within the Src, PLC, IP3phosphorylation of phospho-Src, phospho-PLC, phospho-IP3of abnormally high expression may have some relationship with the CAA incidence of abnormal hematopoietic adhesion mechanism.3. Src/PLC/IP3signal transduction pathway abnormalities may be an important part of the CAA abnormal hematopoietic adhesion pathogenesis.4. Bone marrow nucleated cell intracellular Ca2+concentration was abnormally high expression of these changes may affect the hematopoietic stem cell homing and migration mechanisms.5. Busuishengxue granules can improve the Src/PLC/IP3signal transduction pathway enzymes and intracellular Ca2+expression, make Src/PLC/IP3signaling pathway activation and down the abnormal elevation of Ca2+. The mechanism may be to improve the formation of focal adhesions, regulating the cytoskeleton to promote hematopoietic cell proliferation, differentiation, and improve the hematopoietic microenvironment, thereby improving the homing of hematopoietic stem cells/progenitor cells, migration, and enhance the signal transduction of hematopoietic adhesion hematopoietic function will be restored.
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