The Study On Clinical Effect And Influence On ERK2 Signal Transduction Of Haemopoiesis With Busuishengxue Granule In Chronic Aplastic Anemia

Objective:To observe the clinical effect of chronic aplastic anemia (CAA) treated with Busuishengxue granule(BSSXG) and the difference in expression levels of the enzymes ERK2 before and after treatment.In order to further research the therapeutic effect mechanism about BSSXG treating CAA.Provided the reliable experimental record for seeking a new method for treating CAA and developing a new effective drug to prevent and cure CAA.Method:Sixty cases of CAA were divided into two groups at random, thirty patients of the treated group were treated with BSSXG and thirty patients of the control group were treated with Zaizhang Shengxue tablet (ZZSXT).We observed the clinical effect of the sixty patients treated with BSSXG and ZZSXT.Meanwhile,the expression of the enzymes ERK2of BMSC was detected by Western blot and Immunofluorescence technique before and after treatment.Result:(1) The clinical therapeutic effect was superior in BSSXG group to ZZSXT(P<0.05).BSSXG could alleviate CAA patients anemia symptoms and increase the peripheral blood cells significantly.In BSSXG group,its curative effect was superior in Deficiency of Kidney- YANG type to that in Yin deficiency type(P<0.05).(2) The expression of ERK2 was lower than the normal controlled group before treatment,there are regulated respectively in different degree after treatment,and the Deficiency Kidney-YANG has better regulated degree than the Deficiency Kidney-YIN.Conclusion:(1) The effect of Busuishengxue granule to CAA was superior to that of Zaizhangshengxue tablet.(2) The Abnomal expression of ERK2serum can affect the bone marrow haematopoiesis and is related closely to CAA.(3) Busui Shengxue granule inhibit the apoptsis of bone marrow hematopoietic stem/ progenitor cells by regulationing ERK2 which mediate the signal transduction pathway.Italso can regulation the bone marrow hematopoiesis,increase the proliferationand differentiation of hematopoietic stem/ progenitor cells.(4) The clinical therapeutic effect was superior in BSSXG group to ZZSXT,and the regulated degree of the enzymes Deficiency of Kidney-YIN type were superior to Deficiency of Kidney-YANG type.It also further proves the theory that YANG asthenia is easily treated but YIN asthenia is hard to be cured.