| Objective:In order to make clear the toxic mechanism of Banliuwah,on onehand,we study and forecast the potential toxic mechanism of Banliuwan for Liveraccording to the change of Gene.On the other hand,through tearing apart theBanliuwan to understand the action of each kind of medicine in Banliuwan.Methods:EXPERIMENT ONE: Determine of LD50 of Banliuwan,Liuhuang,LiuhuangBanxia,Liuhuang JiangzhiAnimals and groups:200 mices were randomly divided into 20 groups:five of Banliuwan group,five of Liuhuang group,Five of LiuhuangBanxia group and five of LiuhuangJiangzhi group.Methods of treament:According to the results of beforehand experiment,the Dm and Dn of all each above of groups was found.Because Dm/Dn was four,the proportion in the each group was 1:0.7,and the dose was established.All mices were treated the correspounding dose of 0.5ml for three times in one day.Detection methedsrObservering normal condition of mices for seven days and recording the number of the death of the mices.Statiscal methods:Calculate LD50 and the range of believement of LD50. EXPERIMENT TWO:The study of normal toxic of Banliuwan,Liuhuang, LiuhuangBanxia and LiuhuangJiangzhi.Animals and groups: 140 Wistar rats were randomly divided into 7 groups:normal group, Banliuwan highest group,Banliuwan moderate group,Banliuwan lowest group, Liuhuang highest group, LiuhuangBanxia highest group and LiuhuangJiangzhi hinghest group.Methods of treament:According to the results of LD50,the dose of highest ,normal and lowest group was 1/10,1/50,1/100 of corresponding group.All the rats were treated with medicine for 90 days. Detection metheds: Observering normal condition of rats and examining blood routine,testing liver and kidney function,and meanwhile working up pathology tissue of liver,heart,kidney,head and thyroid gland. Statiscal methods: Differences between groups were compared by F-test.EXPERIMENT THREE:The study of the toxic mechanism of Banliuwan for Liver by GeneAll the process of the experimenr was the same as of the experimen two.when experiment was over,the liver of rats of Banliuwan group and normal group were pick out to make further detection by Toxicology Gene. Result:1 EXPERIMENTONE:The LD50 of Banliuwan, Liuhuang,LiuhuangBanxia, LiuhuangJiangzhi were 20.5314 g/kg,30.4385g/kg,9.7907g/kg and 50.0662 g/kg. 2 EXPERIMENT TWO:2.1 Normal condition:The rats of normal group, Banliuwan moderate group,Banliuwan lowest group and LiuhuangJiangzhi group wereregular.The number of death rats of Bliuwan highest group,Liuhuang group and LiuhuangBanxia group was five,four and five,and meanwhile,the normal conditioan were descend.2.2 Change of Blood routine: The differences between groups were not significantly ( p>0.05 ) .2.3 Change of liver and kidney function: The differences between groups about kidney function were not significantly( p>0.05 ) ,same as among normal group,Banliuwan moderate group ,Banliuwan lowest group and LiuhuangJiangzhi group of Liver function.Of the Bliuwan highest group,Liuhuang group and LiuhuangBanxia group were superior to normal group( p<0.05 ) .2.4 Change of pathology tissue: The pathology tissue of Liver of Bliuwan highest group,Liuhuang group and LiuhuangBanxia group had been spoiled incoordinately. The kidney of Bliuwan highest group has also damanged. 3 EXPERIMENT THREE:The gene about languishment of cell(Crystallin, alpha B; Cytochrome P450, family 1, subfamily a, polypeptide 1. 2 ; Cytochrome P450, family 1, subfamily b, polypeptide 1. 10; Cytochrome P450, 4a10; Ephx2; Flavin containing monooxygenase 4; Gpx2; GSR; COX-2; SOD2; Caspase 1 ; Caspase 8; TRAAD; Bcl2-associated X protein), growth-restrainment and caducity(P21; GADD153; Gadd45; Igfbp6; P53), inflammation(Interleukin 18; Interleukin 1 beta ; NFkB1; Scya21a; Scya21b; MIP-2 (SCYA21)),damangement and restorment of DNA(ATM; ERCC1; XPD; RAD23A; RAD50; RAD53/Chk2; UNG; XRCC1. 2. 4. 5 )were ascended;about oxygenation and metabolize of medicine( Cytochrome P450, family 3, subfamily a, polypeptide 11; Cyto...
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