The Protection Of The Traditional Chinese Herbal Medication Called Fujian Tablet To The BBB In Cerebral Ischemic Stroke

Objective: To investigate the effect of the traditional Chinese herbal medicine called Fujian Tablet on the BBB after cerebral ischemic stroke. Fujian Tablet is ascribed the therapeutic method called"the nourishing of liver and kidney"by Traditional Chinese Medicine (TCM). Methods: Based on the TCM theory that"nourishing the liver and kidney can produce sui"(with sui indicating bone marrow, spinal cord and brain, all related structures in Traditional Chinese Medicine), we set up MCAO rat model to explore the mechanisms of protection that may occur from applying the Traditional Chinese Medicine therapeutic method of Nourishing Liver and Kidney by the use of the Fujian Tablet and observing it's effects of on regulating permeability and structure of BBB. EB was measured by AZO-blue dyeing method to evaluate the effects of Fujian Tablet on the permeability of BBB. Moisture capacity of brain tissue was measured to evaluate the effects of Fujian Tablet on encephaledema. t-PA was measured by euzymelinked immunosorbent assay to evaluate the effects of Fujian Tablet on the fibrinolysin system in order to help prevent destruction of the basilemma. MMP-2 and MMP-9 were measured by euzymelinked immunosorbent assay to evaluate the effects of Fujian Tablet on the gelatinase system in helping prevent destruction of the basilemma. The expression of ET-1 mRNA, ecNOS mRNA and P-gpmRNA in the endothelium were observed through in situ hybridization in order to reflect the effects of Fujian Tablet on the function of the endothelium. cAMP and cGMP were measured by radio-immunity method to reflect the effects of Fujian Tablet on the function of astrocyte activity. TNF-αand IL-10 were measured by euzymelinked immunosorbent assay to evaluate the effects of Fujian Tablet on inflammatory factors. Results: Fujian Tablet can decrease the permeability of BBB and lessen hydrocephalus. Fujian Tablet can restrain the expression of t-PA, MMP-2 and MMP-9 thus protecting the basilemma. Fujian Tablet can decrease the expression of ET-1mRNA and increase the expression of ecNOS mRNA and P-gp mRNA thus protecting endotheliocytes. Fujian Tablet can decrease the expression of cGMP and increase the expression of cAMP to help the astrocytes maintain BBB in co-induction with the endotheliocytes. Fujian Tablet can decrease the expression of TNF-αand increase the expression of IL-10 lowering the inflammatory reaction and helping prevent the destruction of endotheliocytes, astrocytes and basilemma and the resultant further decrease in the permeability of BBB. Conclusions: Fujian tablet with its action of"nourishing the liver and kidney"can regulate the function of basilemma, astrocytes and endothelium in vivo, protecting BBB in order to prevent hydrocephalus.