| The occurrence of diseases may bring huge economic and social losses for the intensive production of livestock and poultry industry. The disinfection of foreign objects (human being, clothes, cage, vehicle etc) entering the poultry farms is one of the important biological measures to reduce the risk of pathogen contamination and prevent the spread of the disease. However, chemical disinfectants often lead to drug resistance, chemical residues and environmental pollution issues. Therefore, it is desired to investigate the effects and mechanism of slightly acidic electrolyzed water (SAEW) disinfection on the surfaces of facilities and human that entering the farm. The finding of this study can provide a new disinfection technique for livestock and poultry farm biosecurity management, and then improve the health and welfare of poultry.The effects of spraying SAEW on reducing bacteria of the different roughness materials (tire, poultry cage) were investigated, and the coupled effects of the cleaning time, treatment time and available chlorine concentrations (ACCs) were also evaluated. The effect of droplet size, droplet deposition amount of SAEW spraying disinfection on the clothes sueface was investigated. The following findings were obtained:(1) The tap water cleaning time, treatment time and ACC contribute to the most important role for the disinfection activity of SAEW, and their interactions on response value were ranked as ACC＞ treatment time>cleaning time. Moreover, the cleaning time was important for the high roughness materials disinfection with the organic presence, the interactions between cleaning time and other factors had a significant effect on the disinfection activity of SAEW (P< 0.05).(2) The treatment of tap water cleaning for 15 s followed by SAEW disinfection for 40 s at an ACC of 50 mg/L resulted in a maximum reduction of 3.12 log10 CFU/cm2 for S. enteritidis on the cage. The cleaning time had less effect on the disinfection of the smooth materials. The interactions among cleaning time and other factors were not significant (P> 0.05), whereas the interaction between treatment time and ACC significantly affected the SAEW disinfection effectiveness (P< 0.05).(3) Droplet size had a significant effect on the activity of SAEW spraying disinfection (P< 0.05). The disinfection effect of the nebulizer with a diameter of 80-90 μm was significantly higher than that of the ultrasonic nebulizer (fog diameter≤ 30μm) (P< 0.05) at the central target region of the spraying. However, the bactericidal effects of nebulizers with a big fog diameter at the different locations were significant different (P< 0.05), but the bactericidal effects of the ultrasonic nebulizer at the different locations were not significant different (P> 0.05).(4) The retention amount in central district was higher than that at the the two end districts at the sprayed target area. With increasing the pressure and pore size of the nozzle, the two end retention increased firstly and then declined. The coefficient of variation rose at the beginning and then reduced with increasing the pore size of the nozzle. The maximum retention amount for SAEW disinfection on the surface of clothes was 1.49×10-2 g/cm2 and the SAEW disinfection effect increased with increasing the retention, but it would not increase when the retention was higher than the maximum retention point.(5) SAEW treatment for 1 minhad a more significant effectiveness on the drug kits and clothes in the disinfection channel without any negative effect on the respiratory of human being compare to the disinfectants of KAS and GS (P< 0.0.5). Therefore, SAEW as a new disinfectant can be used in the disin fection channel in poultry farms. At a same ACC and treatment time, the materal type was also an important factor for the disinfection effectiveness. The inactivation efficiency of SAEW spraying treatment for reducing the S. enteritidis on the the surface of iron materials, drug kits and clothes was different (iron> kit> clothing). |
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