| Object:1. To explore the mechanism and provide experimental basis of traditional Chinese medicine’s treatment in type Ⅲa prostatitis, on the basis of observing the curative effect of the Detoxicating and activating blood Decoction’s treatment in type III A prostatitis and its influence inTNF-α、IL-8、 IL-10and WBC in prostatic fluid.2. To observe the effect of Detoxicating and activating blood Decoction on TNF-α,IL-8,DL-10in rats prostate tissue with chronic nonbacterial prostatitis.Methods:1. The clinical observation:Bring into80type ⅢA patients with chronic prostatitis according to treatment’s sequence, divide into the treatment group (Detoxicating and activating blood Decoction group) and the control group (Cernilton group) randomly, n=40. The treatment group take Detoxicating and activating blood Decoction150ml each, twice per day, morning and evening hours service. The Control group take Cernilton slices each one for one pill and twice per day, morning and evening hours service. The two groups are in a course of treatment with eight weeks. All patients use the NIH-CPSI table symptom score before and after the test. Patients’prostatic fluid were collected and saved before and after the treatment.The content of TNF-α、 IL-8、DL-10in all subjects’prostatic fluid should be detected by ELISA before and after the clinical treatment.2. Animal experiments:selected30male SD rats from the40ones randomly, castrated and injected subcutaneously with estradiol benzoate and caused to be the animal models of chronic non-bacterial prostatitis. The remaining10as the blank control group (normal group). After the modeling, the chronic non-bacterial prostatitis rat model were divided into model group, Cernilton group and Detoxicating and activating blood soup group randomly. The dosage of Detoxicating and activating blood soup group is1g/kg·d (4ml/d), the Cernilton group’s is0.025mg/kg·d (4ml/d),the normal group and the model group were given the same dosage with distilled water. These groups were administered orally, once daily, administered for30d continuously. The rats were killed after weighting at the end of treatment. Take the prostates’ issue to observe their pathological changes, measure the content of TNF-α、IL-8、IL-10in homogenates of prostates’tissue by ELISA.Results:1. Clinical study:①Detoxicating and activating blood soup can treat type IIIA CPPS effectively, improve patients’clinical symptoms significantly, reduce the points of NIH-SCPI effectively, lessen the TCM syndrome score obviously, decrease the content of the WBC in EPS.②Detoxicating and activating blood soup can significantly reduce the levels of TNF-α IL-8in patients’EPS. It has the localized immune function regulation to type Ⅲa CPPS patients.③The levels of TNF-α、IL-8、IL-10in EPS and the score of pain symptom of the NIH-CPSI was correlated positively (p<0.05).2. Animal experiments:①pathological observation show that the pathological changes of the prostate tissue of model group rats are chronic inflammation. The prostate gland generally adhere the surrounding tissue, glands are dark red or brown and its quality are a little stiff. Under the microscope,their performance are mainly lymphocytes and mononuclear cell infiltration, interstitial edema and fibrous tissue hyperplasia, prostate epithelial damage, atrophy and loss, prostate wall hyperplasia or destruction. The statistical analysis of classification of pathological slice of prostate grand biopsy show difference significantly (P<0.01) between model rats and normal rat indicate that the model was successful.②Detoxicating and activating blood soup group rats’prostate epithelial tissue returned to be normal, the wall structural integrity, interstitial edema disappeared. Pathological classification statistics show the difference was statistically significant (P<0.01, P<0.05) among the comparison of the Detoxicating and activating blood soup groups the Cernilton group and the model group.③The levels of TNF-a, IL-8in model group rats’prostate tissue have more increasing than the normal group’s (P<0.01); the levels of TNF-a, IL-8in the rats’prostate tissue of the Detoxicating and activating blood soup group compared with the model group’s were significantly lower (P<0.01),while the levels of EL-10compared with model group’s don’t have difference obviously (P>0.05).Conclusion:1.The treament of Detoxicating and activating blood Decoction can treat type III A CPPS effectively, improve patients’clinical symptoms significantly, reduce the points of NEH-SCPI effectively, lessen the TCM syndrome score obviously, decrease the content of the WBC in EPS, eliminate inflammation and reduce the patient’s pain, which is a recommendable treatment method and Clinical Thoughts.2.Type Ⅲa CPPS’s scores of pain symptom and the cytokine of TNF-α、IL-8、 IL-10were positively related, which can be formulated as the reference index of patient’s condition and treatment effective of type IIIA CPPS. 3. The levels of TNF-α、IL-8、IL-10in prostates’tissue of rat model with chronic non-bacterial prostatitis were increased, which indicate that the cytokines are involved in the incidence of inflammation of chronic non-bacterial prostatitis. We can achieve a therapeutic effect by the mean of its regulation which can alleviate or eliminate the inflammation in the prostates’tissue.4.Detoxicating and activating blood Decoction can reduce or eliminate the inflammatory response of rat prostate tissue, improve the destruction of its organizational structure, which play a significant protective effect on prostate tissue and complement the experimental basis for clinical studies.5. Detoxicating and activating blood Decoction can reduce the levels of promote inflammatory factor of TNF-a and IL-8in prostates’tissue of the rat model, thereby reducing the extent of inflammation in prostate tissue. |
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