Studies On The Quality Assessment And Pharmacodynamics Of Herba Bupleuri

The standardization of quality control criteria of traditional Chinese medicine is theimportant content of industrialization,modernization and internationalization of traditionalChinese medicine.Bupleurum scozonerifolium Wild.(Spring Chaihu), which belongs to Umbelliferaefamily, is one of traditional Chinese medicines in common used with its entire plants.Abounding in natural resources, this species is widely distributed in jiangsu province ofChina. For it is harvested in spring, it is called"Spring Chaihu"or"Yahu"as usual,wasrecorded Chinese crude drug standard of jiangsu province(1989 edition),to develop andutilize the abundant resource, In this Paper, studies on the quality assessment andpharmacodynamics study of Bupleurum scozonerifolium Wild.The paper is composed of three parts.PartⅠis the literature study; partⅡisexperimental study, partⅢis summary and discussion.PartⅠliterature study: Review the bencaological study of Spring Chaihu, researchadvancement of Bupleurum and the standardization of quality control criteria oftraditional Chinese medicine,etc.PartⅡexperimental study:1 Variety certification study: definite the herb is the entire plants of Chun Chaihu.2 Chemical composition study: Spring Chaihu contains saikosaponins a,d andvolatile oils. different regions、different extraction mothed and different gathering seasonscan affect the content of volatile oils.3 Quality criteria study: using the UV and HPLC method, we determin the contentof saikosaponins a, d and total flavones, establishing the HPLC-Fingerprint Spectrum ofSpring Chai hu.4 Pharmacodynamics study: (1)Both North Chaihu and Spring Chaihu can decreaseanimal fever induced by yeast to rat and endotoxin to rabbit, but the potency of springChai hu is lower than north Chun Chaihu’s, There was significant difference betweentreated groups and model groups (P＜0.05). There are good antipyretic effect betweennorth Chai hu and spring Chai hu and,moreover, Spring Chai hu is better.(2)Both the high dosage in Bupleurum chinense DC and the low and high dosage in Bupleurumscorzonerifolium Willd could inhibit ear edema, reduce the abdominal capillarypermeability, whose differences in anti-inflammatory were significant compared withcontrol group (P＜0.05 or P＜0.01). The high dosage in both north Chahu and SpringChaihu could decrease writhing times in mice, whose differences in abirritation weresignificant compared with control group (P＜0.05). The high dosage in both north Chaihu and spring Chai hu could enhance the threshold of pain. Both north Chai hu and springChai hu have showed good anti-inflammatory effects, but the latter is superior to theformer. In addition, both north Chai hu and spring Chai hu have also showed analgesiceffects, but there were no significant differences in analgesic effects between them. Theexperiment of dimethylnitrosamine (DMN)-induced chronic liver injury and anti- hepaticfibrosis in rats of noah Chai hu and Spring Chai hu show: Both north Chai hu andSpring Chai hu have protective action on chronic liver injury and anti- hepatic fibrosis inrats. Hepatic functional parameter were improved to different degrees, can significantlyinhibit the increasion of ALT、AST、ALP and loss of TP、ALB at all dose levels, thecontent of hepatic tissue collagen protein、HA、LN decreased significantly; improvedliver pathological changes; have good effect on anti-liver cell apoptosis; and downregulation the exprission of NF-KB mRNA. north Chai hu is better than Spring Chai huon protective liver action.5 Set up the standard of Bupleurum scozonerifolium Wild.(protocol) base on theresearch result of variety certification, chemical composition and quality criteria study.PartⅢAnalysis and discuss the experimental result.