Study On Purification Of Crocin And Geniposide

Crocin and geniposide consist in the mature fruit of gardenia , having got the extensive application in food processing industry, beverage manufacturing industry, animal feed industry and medicine industry. At present, the production technology of crocin is complex with the low yield and the low colority . Usually the geniposide is general to be exhausted directly with the waste liquid, leading to the resources wasted.This paper studied every kind of factors that affects the yield of extraction of crocin , getting better leaching agent, leaching temperature and leaching method. The experimental results show that better leaching conditions are that the leaching agent is 50% ethanol aqueous solution( v/ v) under the room temperature , leaching time is 24 hours, the ratio of gardenia to agent is 14:1.In this paper .the separation result of the crocin with macroporous adsorption resins by column chromatography was studied. Some adsorption resins were chosen for the purpose of separating crocin. By the experiments on the static unit volume adsorption capacity and the selectivity of every kind of resins for crocin , NKA macropore adsorption resin was chosen as the adsorbent of the crocin. Regarding NKA macroporous adsorption resin as the research object, the experiments on the static adsorption rate of the resin for crocin, the adsorption curve of the resin for crocin, effect of ethanol concentration on the behavior of the resin adsorption, the dynamic leaking curve under the different concentration, the dynamic leaking curve under the different flow rate were made and better conditions of NKA absorbing crocin were got. After studying the wash of the impurity and the product on adsorption column , it was found that after washing the adsorption column by deionized water, sparse alkali liquor , sparse ethanoland, and then crocin was eluted by 85% ethanol aqueous solution( m/ m), the crocin solution was very high easy to be dried, after drying .the colority of crocin is more than 420.An investigation of stability indicated that after adsorption and desorption for ten times, the stability of NKA resin was still excellent.In this paper, the separation result of geniposide with 1300 macroporous adsorption resins by column chromatography was studied. Through the experiments on the static adsorption rate of the 1300 resin for geniposide, the adsorption curve of the 1300 resin for geniposide, the behavior of the resin adsorption in different pH and the dynamic leaking curve under the different flow rate ,better conditions of 1300 resin absorbing geniposide were got. After studying the wash of the impurity and the product on adsorption column , it was found that after washing the adsorption column by a little deionized water and sparse ethanoland, and then geniposide was eluted by 85% ethanol aqueous solution( m/ m).Aafter drying geniposide solution . the purity of geniposide is more than 75%.