Studies On The Extraction And Purification And Stability Of HSYA

The dried flower of safflower (Carthamus tinctorius L.), is a commonhuoxuehuayu remedy in the traditional Chinese medicine, Pharmacologicalexperiments had proved that it had the function of depressing blood pressure,anti-cruor, anti-inflammation and enduring anoxemic. It is an importantchinese medicine to treat gyaechological diseases, cardiorascular diseases,cerebrorascular diseases. A major component in the flower of safflower,which has been found to possess pharmacological effects, is safflor yellow(SY). Hydroxysafflor Safflor yellow A (HSYA) is the main component ofsafflor yellow. HSYA content affects pharmacology of Carthamus tinctoriusdirectly, but at present the document relating to the methods of HSYAseparation have much room for improvement. In this study, the method forthe extraction and separation of HSYA from the flower of safflower wasexplored, which is simple, low cost, no pollution. At the same time, it can beapplied in production.Besides, one kind is more valuable nature pigment, is various a softdrink, cake, candy, food of all kinds and ideal cosmetics colouring agent. It isvaluable in exploiting and using of food industry. But the particular HSYAstructure characteristic affects whose stability. Consequently, we research theeffects of light, temperature, pH, metal irons, and reductant on the stability ofHSYA by HPLC.This dissertation consists of four chapters focusing on the study of theextraction, separation, chemical characters and stability. The concrete workmainly has following few aspects:1. The contents of HSYA was detected through HPLC. To take thecontent of HSYA and extraction ratio of HSYA as the index, the optimalextracting condition for improving the yield of HSYA from SY wereinvestigated. In order to optimize the extracting condition we adoptedorthogonal experiment design (3 factors and 3 levels) to determine the bestextracting conditions. The optimal extraction conditions are extracting temperature 60℃, extracting twice, extracting time 45 minutes and watervolume 30 multiples by using orthogonal experiment design (3 factors and 3levels). In these conditions, the extraction rate of HSYA is as high as14.2mg/g.2. First-step purification of HSYA from safflower was studied by usingthe Macroporous adsorptive resins. The results showed that the Macroporousadsorptive resins enriched HSYA, at the same time could remove a lot ofextraneous components, and obtained a higher content of HSYA product.There are many advantages using this extraction technology: (1) It canremove a lot of extraneous components and can save solvent, time and havehigh performance, higher content products. (2) The used solvents are safe andinnoxious, and do not contaminate environment. (3) Macroporous adsorptiveresins can be used many times and regenerated easily.3. The results showed that using new resin M to refine HSYA fromsafflower was more feasible, effective. The new resin had the sameseparation efficiency as ODS-C₁₈, but had longer life, better stability andbigger sample capacity than ODS-C₁₈. Furthermore it could bear strong acidand strong base, was suitable to the various organic solvent and aqueoussolution, and had quick adsorption speed and broad application range. So itwas especially applied to the separation of natural compounds. It not only canbe used many times and regenerated easily and have high performance,higher content products, but also is good for industrialization production.The purity of samples could reach over 80% when they were separated twiceby the adsorptive resin.4. The effects of light, temperature, pH, vitamin C, the trace metal ironsand EDTA on the stability of HSYA have been studied through HPLC. Theresults showed that HSYA was relatively stable and the light and temperatureonly had slight influence on its stability under acidic condition, while lightand temperature had tremendous influence under the condition of alkali(pH＞7). In addition HSYA could be hydrolyzed easily when the temperaturewas beyond 60℃. Fe²⁺ and Fe³⁺ could catalyze hydrolysis of HSYA, while the trace vitamin C and EDTA could improve the stability of HSYAobviously.