The Study On Preparation Technology Of Aloe Extractum And The Quality Standard

Aloe extract is the extraction of Aloe, which is purified by Macroreticular Resin , Aloe is the concentrated and dry extraction of Aloe Barbadensis Miller, Aloe ferox Miller and other plants of the same category.The modern researches express that tha Aloe has many efficiencies,Such as anti-cancer,anti-inflammation,anti-virus,anti-bacterium and desinsection,antifebrile and liver-protected,strengthen immunity etc.And it was used for various area such as medicine industry,daily use industry,cosmetic and make-up,food and health care etc.So,the Aloe is a kind of medicine plant that has higher economic value.The Anthraquinones and Polysaccharides in A.vera L.Var Chinensis Berg are the main active ingredients for medicine,have efficiencies suchasanti-bacterium,reduce inflammation,purgation,anti-cancer,anti-decrepitude etc. Nowadays,the existing main extraction methods are solvent-extraction method and ultrasonic extraction method,but these traditional extraction methods have defects of low extraction yield,severe pollution,industrialize difficult etc.Up to this point,there has no report of Macroreticular Resin extract aloe,but the feasibility of it has been argued.This paper take the overall yield of Anthraquinones and Polysaccharides as inspection target,studied the technology of extracting active principles ftom aloe by Macroreticular Resin and studied the Quantitative standard of extraction.With Aloin as investigation target the single factor tests and orthogonal experimental design methods L9(34)were applied to analyse the influence of each factor in ethanol consentration,the best extraction condition of reflux extraction:50% ethanol as solvent,ratio of material to liquid 1:15 (ml/g);extract 2 times with 60min each time;the best PH5. The adsorption and desorption capacity of five resins were compared,the preparation of adsorption and purification of Aloe total anthraquiones and Polysaccharides were selected with Aloin as investigation target;Study the absorption capacity of D101,and to optimize the process of adsorption in the pH of sample,adsorption temperature,the sample volume,elution solvent etc.The Aloe polysaccharide enrichment and purification were also inspected.The result shows that the best extraction condition of reflux extraction; 50% ethanol as solvent,ratio of material to liquid 1:15 (ml/g);extract 2 times with 60min each time;the best pH5; The adsorption capacity of D101resin is the best and the stastic exchange capacity reached 16.10mg/g ,ADS-8 resin adsorption capacity of the second , the exchange of ADS-17 adsorption capacity is minimum.D101 resin can be substantially enriched anthraquinones substances in pH4 of aloe extraction; The appropriate adsorption conditions were: concentration of extract 10mg/ml,the pH of extraction 4.0,eluant with 70% ethanol desorption better,the effect of desorption was satisfactory,the desorption rate of static state can reach 84.19%,the best temperature of absorption 20℃;Water as desorption eluant,after enrichment and purification,the content of Aloe polysaccharide reached to 9.51% from 5.45%,up to a certain concentration and purification role.Establish the method-HPLCto determinate the content of Aloin,and study the method.According to the test results in the development of Aloe extract ,the Aloin contention shall be not less 24% by HPLC limit on dry goods.Establish the spectrophotometry to determinate the content of anthraquinone and polysaccharide in Aloe extract,and study the method, the anthraquinone contention shall be not less50%,total polysaccharide contention shall be not less9%by spectrophotometry limit on dry goods.Establish the identification method of TLC,Aloin as reference substance,retained identification methods of Aloe in the《Chinese pharmacopoeia》2005version;study and formulate water should not exceed 5.0%,chi-burning residue shall not exceed0.8%,heavy metals may not be over 10millionths of content limit.In this experiment,the purpose is enriching and purificating the effective site from Aloe , established the enrichment and purification methods with Macroreticular Resin,the research shows that D101 resin is feasible to enrich and purify anthraquinone and Aloe polysaccharides,with good potential applications, and this technologyis applied to industrial production through the test.According to the experimental results,the paper has composed the Aloe extract quantitative standard draft and draft explanation,laid the foundation for the further processing and development of the Aloe's extraction.