Study On Deodorization And Extraction Of Polyphenol From Persimmion Peel

Persimmion belonging to Ebenaceae DiospyrosL is Deciduous tree.It distributes in torrid and temperate zone.It is abundant in flavone,tannis and Carotene etc which are bioactive components,so it is worthy of developing. This paper mainly conducted the extraction,purification and deodorant effect of polyphenols from persimmon peel.The results were showed that:The experiment used the traditional solvent extraction.Based on single factor tests,thogonal test was done.The optimum extraction conditions were that the concentration of acet was 50%,extraction temperature is 55℃,the extraction time was 105min and the ratio of the persimmon peel to solvent was 1:16.Under the optimal conditions the extraction yield could reach 71.16%.Through the absorption of static and dynamic curve of static studying in the six kinds of macroporous resin.Resoult showed that HP-20 resin had excellent absorption and desorption properties.The dynamic absorption conditions were flow speed 2BV/h,column density 2.91mg/mL and pH4.8. Through the research on gradient elution,it showed that:40%ethanol eluted the purity which could reach 32.04%.Through the bacterial experiments,three samples had effects of restraining bacterium.The sample B had better antibacterial effect than the other two samples.The elimination effect enhanced with the concentration of sample increasing.The minimum inhibition concentration(MIC)of sample B on Escherichia coli,Staphy lococus and Saccharomyces cerevisiae were listed as follows:250mg/mL,125mg/mL,250mg/mL.The minimum sterilization quality fractions(MBC)were:500mg/mL,250mg/mL,500mg/mL.Combining apparatus with sensory analysis Method.The results showed that:the extract of Polyphenol had effect on ammonia,trimethlamin,sulfureted hydrogen,formaldehyde and indole.The sample B had the best effect on trimethlamin and the worst effect on formaldehyde.This might relate Polyphenol purity,the structure and character of the smelt substance.Apparatus and sensory analysis methods could show the effect of deodorization exactly.Through the further purification by Sephdaex LH-20,the Polyphenol purity of sample B-2 was 53.64%.The deodorant rate of it on trimethlamine was 62.37%(2mg/mL).Samples were detected by UV spectrum.After compared to the characterristic absorption of polyphenol,the sample was supposed to contain the polyphenol.By using the High performance liquid chromatography (HPLC),we selected three kinds of phenolics standards to determine small molecular phenolics in camelliia chryscath.The result showed that sample B-2 might containe gallic acid,the caffeine and the table caffeine.