Application Study On Extracting And Separating The Herbs Of The Honeysuckle And Gardenia In Qingkailing Injection By Vibratory Shear Enhanced Processing Membrane Filtration Technique

MingXing QingKaiLing injection, a famous product in Guangdong Province, is a traditional Chinese medicine injection which consists of bezoar, Nacre, Buffalo Horn, Milkvetch Root, gardenia, honeysuckle and Isatis Root, with the effect of protecting liver, promoting the restoration of the injury liver cell and so on. For the gardenia, honeysuckle and Isatis Root, their active ingredients'extraction method is water extraction by alcohol sedimentation and heating concentraion, which is time-consuming, has low efficiency, consumes alcohol and energy, costs much money, pollutes the environment and the safety coefficient is low, Morever, the product's quality is unstable frequently. The research uses vibratory shear enhanced processing membrance filtration technology to replace the alcohol precipitation, expecting to save the ethanol, reduct production cycle, improve extraction efficiency, safety coefficient, save the costs and so on.VESP is a new type and high-performance of dynamic membrane filtration and concentraion technology which uses high shearing force on the surface of membrane duing to mechanical high-frequency vibration. Compared with conventional water extraction by alcohol sedimentation and distillation to condense, it possesses a lot of good characteristics. It has a wide range of applications, has strong adaptability and great flexibility while it's procedure of separation and concentration is able to be consecutively carried out, operating at room temperature and does not need to add organic reagents, it is very suitable for separating and purifying unsteady materials. Morever, its production working procedure is fewer, the cycle is shorter, But the efficiency and the security index is higher, the cost and the energy consumption is lower, also the filtrating membranes are comparatively difficult to be clogged and contaminated, membrance life span becomes longer. Quality of products will be promoted as well as fully got assured. The experimental result indicated that conventional water extraction by alcohol sedimentation and distillation to condense and generally static membrane filtration could be substituted by vibratory shear enhanced processing membrane filtration during purification and concentration of the honeysuckle and gardenia in Qingkailing injection. Using VESP and taking the MingXing QingKingLing injection's major components of prescription - honeysuckle and gardenia as the research object, the research respectively extracted, separated and condensed the effective components of them, expecting that can substitute conventional water extraction by alcohol sedimentation and distillation to condense. orthogonal design chiefly applies on the test, taking the valid flux of membrane, relative removal rates of impurity, yield of the chlorogenic acid and gardenia glucoside as index, optimize the main influential factors including pore size of filtration membrane, concentration of physic liquor, pH of physic liquor and the alcohol concentration. satisfactory result were achieved by orthogonal design test. According to preceding optimal parameter array, vibratory shear enhanced processing membrane filtration was compared with conventional water extraction by alcohol sedimentation and distillation to condense through investigating the description, relative removal of impurity, yield of the chlorogenic acid and gardenia glucoside respectively before and after filtration, and confirmed the earlier's test result. Experiment result showed that for the honeysuckle, in the condition of pH=3, solution concentration is 0.05g/ml, using the membraneγhad the best filtration effect, for the gardenia, in the condition of pH=9, solution concentration is 0.05g/ml, the alcohol concentration is 10%, using the membraneγhad the best filtration effect, combined with membraneδof pore size to dehydrate, a process of continuity and high efficiency in separation and concentration of effective ingredient was displayed on us. Experiment result and correlated materials cost audit make clear that vibratory shear enhanced processing membrane filtration strikingly outstrip conventional water extraction by alcohol sedimentation and distillation to condense.Regarded as a most promising high-tech production of the separation technology at the end of the 20th century to the mid-21st centurys, membrane separation technology has become a hot research in the countries of the world. The experiment ultilized the vibratory shear enhanced processing membrane filtration to extract, separate and condense the main active ingredient of the honeysuckle and gardenia in MingXing QingKingLing injection, aiming that can bring this advanced technology of the seperation and purification into traditional Chinese medicine production and substitute widely used the conventional water extraction by alcohol sedimentation and distillation to condense and generally static membrane filtration technique, promoting standardization of membrane separation process and product. Meantime, new theory and technique about membrane separation process is explored and referred to reform old key point of production of the traditional Chinese medicine. In addition, this research will help solve critical issue that limit application of membrane filtration technique in production of the traditional Chinese medicine and contribute our effort to promote modernization of traditional Chinese medicine production.