| Arbuscular mycorrhiza (AM) not only protects plants against environmental, cultural stress and plant pathogens, but also improves plant mineral nutrition, significantly enhances yield and quality. Moreover, as biofertilizers and bioprotectors, it doesn pollute soil and environment. Since arbuscular mycorrhiza fungi (AMF) are obligate symbionts, they must be produced on living plant roots. This is problematic when mass production is needed, which restrict the development and application of mycorrhiza. However, early establishments of the AM status via sowing cultivate mycorrhizal seedlings is advantageous to plant growth. Now the most beneficial effects of AM are obtained in pot culture and sterilized soil. It is still absent the study about these effects under realistic field conditions and unsterilized soil. Therefore this study observed the influence of AMF on the growth and development, yield, fruit quality of cucumber. I The substrate treatments were the mixture of soil/sand (1:0, 1:2, 1:4) and vermiculite. Cucumber seeds were sown with Glomus versiforme and uninoculate. The results showed the infections of AMF were 48.48%-56.15% in mixture of soil and sand. while in vermiculite was 37.82%, lower than soil/sand substrate treatments. The most significant beneficial effects of AM are No.2 substrate treatment (soil/sandl:2), increasing leaf area, dry weight and index of health seedling by 44.09%, 36.46% and 83.62% as compared with uninfected cucumber respectively. Therefore No.2 substrate treatment was the best substrate for mycorrhizal seedling of cucumber .In addition, inoculate AMF treatment in soil also increased the growth of cucumber seedlings. 2 Several different arbuscular mycorrhizal fungi were used to inoculate the cucumber at sowing, after 40 days, transplanting in greenhouse, and observing the influences of AMF on the growth and development, yield, fruit quality of cucumber. The results showed the most of AMF inoculated are able to form mycorrhiza with cucumber. The infections of AMF were from 4 1.74% to 55.69%. GV, GM, GM+GV, GM+GV+GI and GV+GI were the superior fungi for cucumber, which can remarkably improve the growth of cucumber seedling; enhance index of health seedling by 58.14%?23.6%; increase early yield by 21.7%?7.87%; also increase total yield by 19.72%?4.41%, compared with nonmycorrhizal seedlings. The beneficial effects of AMF on leaf area and dry matter weight were gradually decreasing as the cucumber growing. The difference between mycorrhizal cucumber and nonmycorrhizal was narrowing gradually, but the influence of AM on yield, fruit quality and others were still existed. 3 AM also improved fruit quality of cucumber, compared with uninfected cucumber. GM+GV and GV+GI treatments increased Vc content of cucumber fruit by 27.84% and 21.95% ; the effects of GV, 01 and GV+Gl treatments significantly enhanced soluble sugar by 13.29%, 8.25% and 10.20% respectively; GV treatment increased amino acids by 47.66 %, while GM+G1 treatment increased amino acids by 23.19%; soluble proteins were also l7.67%?4.29% higher by inoculating AMF than noninoculated ones. Therefore it need to consider the mixture of AMF in produce and application of mycorrhiza. 4 AMF could enhance photosynthesis of cucumber seedling leaves by 14.58%?5.51%, compared with nonmycorrhizal cucumber, which were major due to enlarging ink?consumption and enhancing leaf area. Cucumber inoculated AMF had 10.53%?2.1 1% higher root TTC reducing activity th...
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