Studies On The Effect Of The Combination Of Chinese Herbs And Colchicines On Liver Damage Induced By Carbon Tetrachloride In Dogs

Twenty-four Pekingese with the age of 1 year old and body weight of 5 kg were used as experimental animals, and divided into 3 groups, the control group (Group A), Colchicines group (Group B) and Chinese herbs+colchicines group (Group C). Carbon tetrachloride was given to the dogs to induce hepatic fibrosis. At the same time, dogs of Group B were administrated with colchicines and the dogs of Group C administrated with Chinese herbs+colchicines to evaluate the effect of Chinese herbs+colchicines on the formation of hepatic fibrosis. The results are as follows:1. At the sixth week, the WBC of Group C was significant lower than Group A, as well as significantly lower than Group B. The results indicate that the combination of Chinese herbs and colchicines has remarkable anti-inflammatory effect, which is better than colchicines.2. Five weeks later after Carbon tetrachloride was given, the serum AST and ALT activity of the dogs in Group A was significantly higer than that in Group C, and the ratio of serum albumin to globulin was lower remarkablely Group C. Suggesting that the combination of Chinese herbs and colchicines can protect liver cell from damage induced by Carbon tetrachloride.3. Compared with Group C, the dogs of Group A and Group B were ineppetite and their bodyweight was significantly decreased indicating that combination of Chinese herbs and colchicines can improve the clinical signs of dogs with liver damage induced by Carbon tetrachloride, and can eliminate the side effect of colchicines.4. The serum SOD activity of the dogs in both group B and Group C were greatly higher, and serum MDA content was significantly lower than in Group A,but there was no abvious difference between Group B and Group C. This results suggest that both the colchicines and the combination of Chinese herbs and colchicines have the effect of antiperoxidation of lipid induced by carbon tetrachloride.5. In this trial, from the fourth to seventh week, the serum hydroxyproline concentration of the dogs in group C is significantly higher than in group A(P<0.05), and the collagen content in liver tissue was significantly lower than group A (P<0.05). There have no abvious difference between the Group B and Group C. The results shows that both the colchicines and the combination of Chinese herbs and colchicines can delay the producation of hepatic fibrosis by decreasing the compose and increasing the decompose of collagen to reduce the deposite ofcollgen in the liver.