Study Of New Form Of Prepared Traditional Chinese Herbal Medicines For Dairy Cow Mastitis

The pathogenic bacteria that caused mastitis were isolated from the milk of the dairy cows with clinical mastitis and submastitis, which were tested by CMT and B.T.B. methods. The pathogenic bacteria were grown to blood-agar culture medium, Chapman's culture medium, MacConkey's culture medium, and Edward's culture medium. The pathogenic bacteria could be classified into Staphylococcus, Streptococcus, Enterobacteriaceae bacteria and the others by the specifical biological chemistry tests. The traditional Chinese herbal medicines were selected on the basis of the inhibition to the pathogens. And all of them were used to make four examinational prescriptions. To decide the final examinational prescription, which was used to treat the mastitis in clinical examination, all examinational prescriptions were tested by its activity against pathogenic bacteria. It was proved that the diameters of the bacteriostasis rings of the Traditional Chinese herbal medicines were larger than 10mm. And the diameters of bacteriostasis rings of the prescriptions were 16.09mm. In vitro sensitivity test showed that the MICs were 0.030±0.018mg/ml.After being removed the heat renders by the active carbon, the traditional Chinese herbal medicines were made up into two kinds of preparations, which were injected intramammarily and inunctated on the udder respectively. In order to increase the activity of the traditional Chinese medicinal preparations against pathogenic bacteria, 10 percent of propylene glycol and 2 percent of azone were added. At the preceding day and the 1st, 3rd, 5th,and 15th day after treatment with examinational prescription, the concentrations of Lactate-dehydroxgenase, Serum albumin, and Alanine-amido-transaminase of the milk from the dairy cow with mastitis were tested. At the 3rd day after treatment with inunctions, the concentrations of SA and ALT of milk decreased to the normal level. The concentrations of two enzymes of the preceding day were different significantly from those of the 3rd day (P<0.01). After being injected intramammarily, the concentrations of SA and ALT inmilk did not change distinctively. We could not make sure the effect of GongYingSan, a comparison medicine, on SA and ALT, as the concentrations of two enzymes fluctuated when we treated the mastitis with GongYingSan. It was proved that all examinational prescription had litter effect on the concentration of LDH (P>0.05).