Study On Contents Of The Total Coumarines Of Standard Cultivated Qian-Hu (Peucedanum Praeruptorum Dunn. And Peucedanum Decursirum Maxim.) In Different Collecting Times

Qian-Hu is a kind of common Chinese herb. There are two species in CP-2000:Peucedanum praeruptorum Dunn. and Peucedanum decursirum Maxim. Their part is the root and main biological active compeonents are coumarines. Since 1960s , there have been more reports on chemical constituents and pharmacology of P. praeruptorum Dunn. and P. decursirum Maxim., but no study on their collecting time. In order to determine the best collecting time of standard cultivated Qian-Hu, we made the study : 1. We used an ultraviolet-visible spectrophotometry(UV) assay method to determine the content of total coumarines in Qian-Hu. The recovery was 99.16% with RSD less than 1.55%. The method was simple,accurate and reproducible. We determined the contents of the total coumarines of standard cultivated P.praeruptorum Dunn. and P. decursirum Maxim. in different collecting times by the method. The contents of the total coumarines increased with growing of the plant. According to the results of statistics, and growing period of P.praeruptorum Dunn. and P. decursirum Maxim.,we concluded that the best collecting time of the standard cultivated P. praeruptorum Dunn. and P. decursirum Maxim. was November.2.To study the determination method of the fingerprint of Qian-Hu and to establish high performance liquid chromatography (HPLC) assay methods separately to determine the fingerprint of the standard cultivated P. praeruptorum Dunn. and P. decursirum Maxim. The results of stability experiment, accuracy experiment and reproducible experiment were accurated. We separately determined the area of total common peaks in HPLC fingerprint from different gathering time of the standard cultivated P. praeruptorum Dunn. and P. decursirum Maxim. The relative retention time and the ranges of relative area of the common peaks were determined. The results showed that the regularity of change of the area of total common peaks was similar to that of the contents of total coumarines of the standard cultivated P. praeruptorum Dunn. and P. decursirum Maxim.in different collecting time.Because the methods were selective and reproducible, the fingerprint could be used for the quality control and indentification of the standard cultivated P. praeruptorum Dunn. and P. decursirum Maxim.3. We also determined the samples from different collecting areas. From the HPLC fingerprints of nine Qian-Hu samples from seven regions,we found that there was a difference in the chromatography peaks.The contents of the total coumarines of the standard cultivated Peucedanum praeruptorum Dunn. and Peucedanum decursirum Maxim.were much higher than those of the other Qian-Hu samples. The contents of the total coumarines of the P. decursirum Maxim.were much higher than those of P. praeruptorum Dunn. We could conclude that the primary plant of Qian-Hu for sale and collecting time were confused .So we might carry out GAP.