Studies On Tissue Culture Of Anthurium Andraeanum

Leaves and stalks are used as explants in the studies on the effect of different factors on nurture in vitro of various cultured varieties of Anthurium andraeanum Lindl.The content of result includes light , different macro-element portion , cutting way of expiants ; these factors affect inductive rate of callus.The results are as follows :Callus should be inducted under darlmess. The best inductive effect belongs to 1/2MS C ( which is the basal media ameliorated from 1/2MS). 1/2MS C+H2 has better effect on four cultured varieties.1/2MS C+H2 ( Bl ) has better effect on adventitious bud's proliferation OP four cultured varieties. Liquid media same as Bl but Agar powder not added , which used to subculture callus , made adventitious buds proliferated on the callus numerously.Rooting culture is better on the media l/2MS+NAA0.1mg/L (R1 ).Using mixture ( cocos nut powder : sand : pearl rock = 1 : 1 : 1 ) as basal matter , paying attention to maintain temperature and humidity , transplanted plantlets are all lively.The research that the whole course about proliferation of 'Arena' 's plantlet , which is cultivated by method of tissue culture without bacterium , was undertaking at the same time.1/2MS C+BA1.0mg/L ( AN3 ) has the better effect on callus induction as well as proliferation of adventitious buds. Liquid shallow layer static nurture can also proliferate numerous adventitious buds.Otherwise , the research of restraining explants' browning has been done in limited level. Disinfecting way of explants , cutting way of explants , PVP all have effect on explants' browning. The research should be done deeply.