Tissue Culture And Rapid Propagation Technology Of Anthurium Andraeanum

Four varieties and 3 different explants of Anthurium andraeanum were used as a source of initial explants in this study .We studied on the effect of factors which effected the tissue culture ,such as selected explants,pre-sterilization ,the basic medium ,hormone and plant growth regulator during the stage of initiation culture,the bud induction ,rooting and also studied the factor effect plant growth regulator such as illumination condition, coconut milk and other factors. Then set up a regeneration system of ahthurium andraeanum by using the technology of tissue culture for factory production.The mains results are as follows:1.The best method for sterilize of explants: it was dip in 70% ethyl alcohol for 30s ,then agitating in a solution of 0.1%HgCl2 for 6 min for leaf, for 8min for stem and stalk, and finally rinsed five times with sterile distilled water.2. The effect of different selected material on the callus induction was different. The stalk for the callus rate was the highest, followed was the stem, but the leaf was the lowest. The times of select explants, when the new leaf extend two weeks, the callus of leaf, stalk was best, its rate was the highest, and the induction time was the shortest.3. The medium: the best basic medium for callus induction was MS. and the suitable growth hormone combination and density was 6-BA2.0mg/L+2,4-D0.2mg/L. With hormone combination and density 6-BA2.0mg/L+NAA0.25mg/L,the bud could grow well and accelerate the multiplication. And with 8mg/L coconut milk, the bud can get stronger.4. The glucose was better than the Sucrose to the callus induction .The rate of callus induction with the glucose was obviously higher than the sucrose.5. Illumination was better than darkness with the callus induction and the bud growth, and with strength of illumination for 1500lux-2000lux natural diffused light was better. But the darkness was bad for the callus induction and the bud growth.6. The rate of callus induction with the 4 different varieties for Anthurium andraeanum was different .It possible concerns with the gene of the variety.7.In subculture, choosing shoots to inoculate on the medium, which make the effect of multipalcation was evident.