Introduction And Tissue Culture Propagation Of Raspberry And Blackberry

Raspberry and Blackberry are a kind of bacca plants, belonging to Rubus of Rosaceae, and it is an important industrial tree for its fruit. It has a good ecological adaptability and abundant nutrition in the fruit i.e. the contents of VE and SOD are the highest of all fruits. So Raspberry and Blackberry are called the third generation fruit trees. Six species of Raspberry and Blackberry from America and Shenyang of China was as the materials for the experiment. The result of introduction and domestication of Raspberry and Blackberry indicates that Tulameen, Willamette, Russia red, Black buttee and Boysen are all adapt to the Yangling region and get a success. Then set up a regeneration system of Raspberry and Blackberry by using the technology of tissue culture, and find a successful propagation system for factory production. So the plants will be taken into the market greatly and rapidly. The main results show that:(1) Tulameen,Willamette,Russia red,Boysen and Black buttee have the better ecology and biology characters, and adapt to cultivate in the region where the ecology condition just like Yangling in north west of China.(2) The time of fruit mature of Raspberry and Blackberry is longer, pick the fruit as soon as maturate and cold storage or machining on time.(3) The tresses of Summit can have fruit in the first year, and its excellences are earlier fruit and fruit long growth time. But in the fruit time there is a phenomenon that some tresses wilt in fruit time, because it can not resist the high temperature in summer, so its proper growth region should be extended to the north.(4) In March, the axillary buds cultivated from tresses in lab as explants, the pollution rate is lowest; but the stem with bud as explants, the pollution is the highest in October.(5)The inducing rate of buds is the highest on the No.Ⅱmedium (MS+BA1.0+NAA0.1) of three different combinations of concentration of BA and NAA, and this combination can satisfied all species of Raspberry and Blackberry.(6) On MS medium supplied different concentration of BA,KT and NAA to induce multiplacation of buds. The results showed that :on MS medium supplied KT2.0mg/l and NAA0.2mg/l for subculture, the multiplacation effect is a best one.(7) The more of subculture time, the higher of coefficient of multipalcation.the coefficient increased rapidly in five generation, then the coefficient not increased more like before or decreased after five generation.(8) In subculture, choosing shoots to inoculate on the medium, which make the effect of multipalcation is evident.(9) On MS medium supplied GA3 2.0mg/l, GA3 can promote growth of shoots, and make an effective rejuvenation cultivation.(10) The rooting rate,the No. Of rooting and the rooting length of all kinds of species are the highest in 1/2MS+NAA0.1mg/l. W is easy to root. The rooting rate of X is lowest and it is difficult to root.(11) The rooting shoots must be trained for 5～6 days in lab before transplanting and then transferred into mixed media with 20℃average temperature and 70% humidity in the greenhouse. The survival ratio is the highest in the mixed media.(12)The tissue culture propagation technology of Raspberry and Blackberry had got the patent.No.2000KG-B08...