| Prunus humilis, a species of Cerasus and originate in china,is cold-resistant, able to bear the drought,saline,alkalinly and barrenly, the wild fruit tree with very strong adaptability. The major object of this study is to exploit the technique systems of tissue culture and rapid propagation for Prunus humilis, through the randomised block design, combine analysis of variance, using the stems as the explant, and the optimal medium and culture programe are studied. The major results were as following: 1.Using the fresh stems of Prunus humilis as the explant for initial culture, the best season for selecting material was middle ten days from 5 to 6 kind soon. The germination ratio was high and less pollution. 2.The optimizing medium for stems initial culture was L+1.0 mg/LBA+0.5 mg/L NAA. The germination ratio was high with seedlings fast grow. 3. In subculture multiplication, the optimizing medium was L+0.5mg/LBA+0.3mg/L NAA.And high times of propagation, the tendency was prosperous that the plant grows. Suitable NAA density was 0.1-0.5mg/L, the density of BA was 0.5-1.0 mg/L, And the value of BA/NAA was useful to hyperplasia of the test tube shoot among 1-10,the period of subculture was suitable for 35 days, the suitable density of inoculation was 10 / bottle. 4.The optimizing medium of rooting was L+0.5 mg/LIAA+3%sugar, the ratio of rooting was high, with strong and quick-grow roots. With open wide bottleneck add to smelt shoot method suitable for relatively distilled water shoot that take root, While transplanting, we could choose vermiculite or nutrition that market sell soil host, the transplant survival rate was high. 5.In simplifying the test of the medium, white sugar, tap water and glass container, could replace and the A.R. sugar, distilled water and triangular bottle, and did not have remarkable influence on the test tube shoot after the substitution.
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