Establishment Of Regeneration System Cultured In Vitro Of Syringa Dilatata Nakai.'Lacera'

Leaves of Syringa species natured in China is rarely cleft, except S.×laciniata, Syringa×persica Linn., Syringa protolaciniata P. S. Green & M. C. Chang, Syringa pinnatifolia Hemsl., and above these species are pinnate or heterphyllous, small split. Syringa dilatata Nakai.'Lacera' was discovered in Heilongjiang Province in 1980s, the feature of large split is rarely distributed in our country, it has high qualities of large and luxuriant leaves and flowers. But the number of this species is few at present, and the maturity of seed is low, seeding plant can't keep its high quality, rooting is too difficult and slow in cottage, so it's difficult to amplification.The purpose of studying of the technology of tissue culture is to use this species to landscape quickly and conserving resource.The major object of this study is to exploit the technique systems of tissue culture and rapid propagation of Syringa dilatata Nakai. 'Lacera'. Using the fresh shoots, leaves as the explant, the optimal medium and culture programe are studied. The major results as following:1. Using the young stem segment, latent bud, leaves and petioole of Syringa dilatata Nakai.'Lacera' as the explant for initial culture, the best explant is stem with axillary bud. The optimizing medium for initial culture is MS+BA3.0mg/L+IBA0.1mg/L. The germination ratio of axillary bud is 98%, average height is 81%.2. The optimizing medium for inducing adventitious buds through subculture is MS+BA7.0 mg/L+IBA0.05mg/L, inductivity is 81%.The genesis pathway of these adventitious bud is from caudex without calli. These adventitious buds can grow with apical bud and form shoots. They can root after transferred to medium of rooting.3. Put the stem on medium (MS+BA0.5mg/L+NAA0.01mg/L) to culture about 30 days, adventitious buds can be induced directly from stem, but these adventitious buds are difficult to straight growth, so it can't be subculture.4. The optimizing medium for callus of leaves is MS+BA1.0mg/L, calli ratio is 100%, calli is good, fresh, green and crisp.5. The optimizing medium for callus of petiole is MS+BA2.0mg/L+NAA0.1mg/L, calli ratio is 100%, calli is good, distributed in bilateral and global of petiole.6. The optimizing medium for callus of stem is MS+BA0.5mg/L+NAA0.4mg/L, calli ratio is 100%, the color of calli is kelly.7. The optimizing medium for rooting is l/2MS+IBA2.0mg/L, the average ratio of rooting is 78%, average radical number is 4, root system is hairchested and grows rapidly.8. The culture under dark and the culture under light have no obvious influence for callusof Syringa dilatata Nakai. 'Lacera'.