| Mirabilis himalaica(Edgew) Heim. as a kind of traditional Tibetan medicine, its apply has a long history with the functions of warm the stomach, tissue regeneration, discharge calculus and diuretic. At present, more than 10 species of Tibetan medicines contain this medicine. Mirabilis himalaica has been widely applied and was in big demand and its was the first Tibetan medicine realize manual cultivation. However, during the application and popularization process, there still has a great deal of obstacles, such as scarce resources, breed confusion, the indistinctness of its substances, and its quality assessment is not comprehensive. To address the above issues, carrired out a study to compare the quality of Mirabilis himalaica from various regions and different growth mode were colleted. All of this is aims to perfect the criterion evaluation and promote the standardization of the cultivation techniques. It will be further to boost the development and utilization of Tibetan medition. In this study, we conducted experiments in herbal textual research, microscopic identification, assay the chemical components and establish the HPLC fingerprint of Mirabilis himalaica. The specific methods and results are as fellows: 1 Herbal textual researchDocuments of Mirabilis himalaica were consulted and classified. The article tries to respectively do the research from the name, morphological characters, distribution, habitat, property, flavor, meridian distribution, harvesting and its processing. Then analyzing the similarities and differences of Mirabilis himalaica and Mirabilis jalapa. It is concluded that the Mirabilis himalaica should belong to Mirabilis jalapa Linn. The result in accordance with the record of Flora. 2 Macroscopical and microscopical identificationFrom the study on morphological and histological, we find the difference on plant amorphous, material character, micro-character of Mirabilis himalaica. There are significant differences in morphological characters.It mainly displays in section color, concentric rings, quality and smell. In the transaction section are not same. Mainly displays in cerk layer, cortex, vascular bundle and primary xylem. But there are no significant differences in powder microscopical identification.3 Isolation and identification of the chemical constituentsDired Radix Mirabilis himalaicae, got grinded, was extracted under 75℃ in 75% methyl alcohol and concentrated. Then the ethyl acetate and petrolun ether were chromatographed on silica gel column and sephadex LH-20 column, respectively. Atotal of 8 compounds are isolated, identified as tricosane acid,β-sitosterol, Mirabijalone A, Boeravinone C, Mirajalone I, Abronione, Mirabijalone H and Boeravinone F. Among these compounds, tricosane acid, Mirabijalone A and Abronione is very effective anti-tumor agent. This suggests the potential in antitumor of Mirabilis himalaicae. Mirabijalone H and Mirabijalone I for the new compound, its pharmacological activity needs further research. 4 Determined the chemical compositionBoeravinone C, MIrabijalone A and Mirabijalone I as index which have very high content with high activity. Determined the content of three compounds by HPLC. Compared the average contents of three components of wild in Gansu, wild and cultivated in Tibetan. The results show that wild in Tibetan than cultivated in Tibetan than wild in Gansu of Boeravinone C, but MIrabijalone A and Mirabijalone I is wild in Tibetan than wild in Gansu than cultivated in Tibetan. Using SPSS 17.0 software to analysis of variance, in different regions, there was no significant difference between cultivated and wild. 5 Established HPLC fingerprintHPLC fingerprint of Radix Mirabilis himalaicae was established,9 common peaks are indicated and 6 chemicals are classified. By comparison, we find that Mirabilis himalaicae in retention time between 17 to 40 minutes have 8 common peaks. Therefore, it is preliminarily confirmed that 17 to 40 minutes as feature regions of Mirabilis himalaicae. |
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