| Objective:This research was aimed at observing the effect of cluster needing of scalp acupuncture on the neurological function score and the Synaptonphysin P38 expression of rats with cerebral ischemia after MCAO model, and studying the mechanism of cluster needing of scalp acupuncture on cerebral ischemia rats’ neural recovery.Method:The experiment selected 48 healthy male SD rats, and divided them into 6 groups in a random way:7d cluster needing group (group A-7d),14d cluster needing group (group A-14d),7d model group (group B-7d),14d model group (group B-14d),7d sham operation group (group C-7d),14d sham operation group (group C-14d). Group A and group B were made by middle cerebral artery occlusion (MCAO) with improved monofilament. Evaluated the nerve function on the 24 hour post the operation and 1h before collecting the brains in every group. The rats in group A were treated with cluster scalp acupuncture everyday, and no treatment in group B and C. Collected the brains and tested the expression of Synaptonphysin P38 in cerebral ischemia rats by Springbio method.Results:1. The data of the nerve function score prompt that the differences of the score in 24h after the model preparation among group A, B and C were statistically significant (P<0.05), indicates the models were successfully made. Among them, in terms of the score of group 7d at the 7th day, group A was better than group B, and the differences was comparable (P<0.05). In terms of the score of group 14d at the 14th day, group A was better than group B, and the differences was statistically significant. Statistical analysis showed that cluster needling of scalp acupuncture for cerebral ischemia rats has favorable effect on neural recovery.2. The data of Synaptonphysin P38 expression indicates that at the 7th day, the average expression of Synaptonphysin P38 of group A was significantly higher than group B, and the differences were statistically significant (P<0.05). At the 14th day, the differences among group A and B were not statistically significant(P>0.05), but the average expression of SynaptonphysinP38 of group A was still higher than group B. Statistical analysis showed that cluster needling of scalp acupuncture for cerebral ischemia rats can improve the expression of Synaptonphysin P38.Conclusion:1,Cluster needling of scalp acupuncture can reduce the score of the neurological function in cerebral ischemia rats, and it has favorable effect on neural recovery.2. Cluster needling of scalp acupuncture can improve the expression of Synaptonphysin P38 in cerebral ischemia rats, and it indicates that cluster needling of scalp acupuncture can promote neural recovery.
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