Studies On Tissue Culture,Artificial Seeds And High Performance Liquid Chromatography Fingerprint Of Pinellia Ternata (Thunb.) Breit.

Chinese traditional medicine Pinellia ternata (Thunb.) Breit.'s tissue culture has been systemically studied in this thesis. Meanwhile, the process,endosperm and storage condition on artificial seeds were studied. Besides, the chromatographic fingerprint of Pinellia ternata (Thunb.) Breit. was established by applying HPLC and analyzed the extracts of distilled water from wild tubers root in different regions. The main results of experiment are as follows:1. The rapid propagation system has been established by callus culture. Young leaves and petiole were both cultured on several inducing MS medium, which supplemented with different concentration of growth regulators, such as 2,4-D/KT, 6-BA/IAA.The results indicated that the base of petiole of Pinellia ternata (Thunb.) Breit. could be induced to form callus with high growth rate on the MS medium containing 2,4-D 0.2 mg/L and KT 1.0 mg/L. The optimal inherit era culture medium of calli was MS medium containing 2,4-D 0.5 mg/L, KT 1.0 mg/L, ABA 0.5 mg/L and AC 0.4 g/L. This medium could enhance the growth rate and reduce the brown rate of call.2. By the study on calli culture and differentiation on medium supplemented different concentration of PP333 and B9, the results indicated that PP333 was the effective exterior conditioner, could make plantlets in vitro of Pinellia ternata (Thunb.) Breit. strong. Various biologic characters of planlets in vitro on medium supplemented with different concentration of PP333 were compared, and the result indicated that low concentration of PP333 favored the planlets in vitro grow stocky, and high concentration of PP333 restrained the natural growth of the planlets in vitro. The best stocky medium was MS medium with 2,4-D 0.5 mg/L, KT 1.0 mg/L and PP333 0.6 mg/L. The effect of chosen concentrations of B9 on calli culture and differentiation was not remarkable.3. Basic process and the condition artificial seed capsule, artificial endosperm, semination and storage of artificial seeds of P. ternata. were investigated. The results demonstrated that optimized process of making artificial seed should be as following: About 2-3mm size tuberlets from callus were cut, and cultured tuberlets were encapsulated in 3% solidum alginate in 1/2MS solution and then dropped in 2% CaCl₂ solution for 15-20min. Artificial seeds were seminated on culture utensil...