Analysis Of The Molecular Fingerprints For Major Cultivated Strains Of Auricularia Auricula

Analysis of molecular fingerprints on main cultivated strains of A auricula were studied. The genetical diversity was inpersonally evaluated and methords of distinguishing different cultivated strains were found out. Those were the basis of protecting and utilizing germplasm, breeding, property right safeguarding,reinforcing managing of A auricula. Thirty-four mainly strains of A. auricula which were cultivated in China were selected in this study. Their molecular fingerprints and genetic similarities were analyzed by their esteraseisozyme,ISSR,SCRA and SRAP.The main results were as following:1 There are 113 isoenzyme bands among 34 strains revealed by the results of esterase isozyme zymogram. The number of isoenzyme bands of each strain was two-six bands, most strains had two-four bands ordinarily. There were 20 zymotypes among 34 trians tested, some showed the same zymotypes. The 34 stains were divided into 3 groups at certain coefficient level. The similarity coefficient of some strains were very high, so it indicated that their genetic similarities were very close, and they may be synonym.2. Based on the optimization Of amplification system by Orthogonal design, 13 primers selected were used to amplify genomes of 34 strains tested. A total of 129 bands were amplified, and 96.9% of which were found to be polymorphic, with 9.9 bands per primer in average. Model figure of ISSR fingprinting were constructed and computerized by better 32 ISSR bands selected. It also confirmed that ISSR technology could be used to detect and identify A auricula strain fast and accurately.3. 8 strain-specific bands from 6 strains have been gained by the analysis of ISSR zymogram. And two specific bands from strain 173 (Primer4 ) and 186 (Primer 16) were excised from agarose gels, cloned and sequenced. Based on the nucleotide sequences, primer pairs were designed and synthesized, and developed successly SCAR marker. These SCAR markers provide a new preciser and rapider way for strain identification of A. auricula.4. 11 pair SRAP primers selected were used to amplify genomes of 34 strains. A total of 154 bands were amplified, and 148 of which were found to be polymorphic, with 14.0 bands per primer in average. The result of clustering was similar to the result of ISSR, which showed that SRAP could also be an effective technology in the analysis of molecular fingerprints of A. auricula.5. The analysis results of the two DNA fingerprints showed, the 34 strains were divided into 3 groups at certain coefficient level: the strains of I group mainly came from Notheast area,and included a few Hennan's and Beijing's strains;the strains of II group...