Suspension Cell Culture And Changes In Resveratrol Content From Polygonum Cuspidatum

To study on a new industrialization pathway that some plant cells produce resveratrol (a kind of secondary metabolin), the Polygonum cuspidatum cells were used as a research object in this paper. The foundation and optimization of a suspension culture system were acted as the aim. Based on some single factor tests and the orthogonal trials of four main factors, Polygonum cuspidatum callus were induced, suspension cell system were preliminary erected and suspension culture medium and condition were optimized.The leaves, meristem, and stems from polygonum cuspidatums were used as explants to induce calli and subculture in medium with different plant hormone combination; The relaxed calli was used to erect suspension cell system, and then the resveratol content was determinated by using HPLC.The results were indicated that, the calli development rate from meristem inducement was lower than that from leaf (maximum100%), and stems(maximum97%).The inducement medium were as follow, respectively:for the leaf, MS medium with6-BA2.0mg/L+2,4D4.0mg/L, and for stems, MS medium with6-BA2.0mg/L+2,4D4.0mg/L, and subculture medium both were MS medium with6-BA2.0mg/L+2,4D4.0mg/L.Through single factor and orthogonal experiment, this study ascertains the best condition of polygonum cuspidatum cell suspension culture was that during cell suspension culture,2mL of Chloride (1M) at the3rd day and4mL of sucrose (1M) at the9th day were added into the medium, respectively; at subculture,60%inocula were maculated to fresh medium; in all suspension cultures, the temperature was at25℃, the shaking spreed was120r/min. At that time, the content of resveratrol was63.5μg/g, and the dry cell weight was45.5mg/mLIn this research, screening the explants of Polygonum cuspidatum, getting the relaxed callus and optimizing cell suspension culture medium and condition, it will provide industrialization production of resveratrol to accumulate preliminary paramete...