Study On The Biological Characteristics Of A Resveratrol -Producing Fungus B-39 And Dual Culture Of Fungus And Suspension Polygonum Cuspidatum Cell

In this paper, the fungus B-39 was offered by Key Laboratory for Forest Products and Chemical Industry Engineering of Hunan which could produce resveratrol. Its extracts were analyzed by TLC and HPLC and identified as Penicillium chrysogenum based on morphological taxonomy. The cultural optimum conditions and the resveratrol-accumulating characteristics were studied and the results showed that the cultural time, temperature, pH and inoculums had more influential. The optimization of cultural substrates and conditions were suggested by one- factor and orthogonal tests that the optimum cultural substrates were: saccharine 20 g/L , NH4NO3 2 g/L , the optimum cultural conditions were : pH 6.8-7.0, 28℃, 7 days , initial inoculums 10% and 100 r/min. On the equal content (0.5%), Mg²⁺ could evidently increase the resvertrol content, Zn²⁺ also had some effection. 3 mmol/L Phe affected on the growth and the resveratrol content. The growth and the resveratrol content of B-39 respectively were 4.9570 g/100 mL and 8.6617μg/100 mL on compositive fators.Observed the conformation and growth patterns of the fungus B-39 and suspension Polygonum cuspidatum cell by the naked eyes and optical microscopes, and inspected the resvertrol accumulation features by HPLC, the results showed: they were normally grown and promoted each other in the co-culture system, and a dynamic balanceable system may be established. When inoculums was 0.2%, the growth of the co-culture was the best. This presumably due to the fairly competition of each other , namely the balance of the abilities of occupied space and food. The co-culture time had evidently affected on thr growth and the resveratrol content of the co-culture and had some periodicity and rules. These provided the basis for the study of the co-evolution of plants and micro-organisms, and for the mechanism of symbiotic patterns.