Screening Of Injectable Chinese Herb Medicine Solutions And Its Prevention And Cure On Mastitis Of Dairy Cattle

Dairy cows mastitis is one of the most common disease in cow cultivation. It not only influences quantity of cow milk production but also creates the serious economic loss. It has been paid close attention to prevention the cow mastitis. At present the cow mastitis of the main pathogenic bacteria are Staphylococcus aureus, Escherichia coli, Streptococcus agalactiae and Staphylococcus dysgalactiae. Usually the cow mastitis are cured with antibiotics, however, with more and more antibiotics were used, the problems of antibiotic residual and bacterial resistance are becoming more and more serious. Chinese herbal medicine does not yield tolerance and have the nature of low poison, anti-inflammatory and so on.Six Chinese herbal medicine were choosed as the experimental drugs. According to the results of the inhibitory activity of singal drugs and its compound on bacteria in virto and anti-inflammatory trials and binding Chinese Veterinarian themry and Chinese herbal medicine pharmac themry, the optimal constitution of Chinese herbal medicine compoud were determined, and parenteral solutions of which were made. At the same time, reliability and clinical therapeutic action of Chinese herbal medicine compound were studied.The trial and results are as follows:1.Inhibitory activity on bacteria in virtoThree bacteria (Staphylococcus aureus,Escherichia coli,Streptococcus agalactiae) were isolated and identified from the milk of the cows infected with mastitis. MIC and MBC of Chinese herbal medicine on the three bacteria were investigated by using of test tube double dilution.The results showed that the antibacterial activity of Herba Houttuyniae on Escherichia coli were stronger relatively, and their MIC reached 62.5g·L^-1. The antibacterial activity of Flos chrysanthemi indici, Fructus forsythiae, Flos lonicerae on Staphylococcus aureus were stronger, and their MIC respective reached 15.7 g·L^-1,31.3g·L^-1 and 31.3g·L^-1. The antibacterial activity of the fourth trial of Chinese herbal medicine compound on three pathogenic microorganisms was stronger relatively than others.2.Anti-inflammatory experimentAn anti-inflammatory experiment of injectable Chinese herbal medicine compound solutions was designed by swelling of mice pinna by xylene.The results indicated that the anti-inflammatory action of Chinese herbal medicine compound were stronger, and exhibited a significant inhibition on swelling of mice pinna by xylene. The...