Establishment Of Tissue Culture And Cell Suspension Culture Systems Of Abelmoschus Esculentus L.

Abelmoschus esculentus L. is an annual herbage plant in the Mal Vaceae family. It is used as vegetable,Chinese traditional medicine and ornamental plant,which has very high nutrient, medicine and ornamental value. As people come to know more about Okra,the applied prospect is getting more and more vast, which increase demand and perform good in market. And tissue culture technique of Okra has been given even more attention. Obtain good material and reduce the cost by tissue culture, which used as references for the usuage of Okra. A regeneration system of tissue culture in vitro including embryogenic callus induction and embryogenic susppension using stem as explant was established on Abelmoschus esculentus L. The main results were as follows:1 Somatic embryogenic calli of Abelmoschus esculentus L. was produced. For the stem, 75% ethanol treating for 30 seconds first and 0.1% mercuric cholride treating for 8 minutes could be the best design of sterilization combination. Somatic embryogenic callus was induced from stem on MS medium supplemented with 2.0 mg·L^-12,4-D and 3.0 mg·L^-1 ZT. 2,4-D was the necessity phytohormones. The effect of phytohormones,nitrogen and light on embryogenic callus formation was discussed.2 Somatic embryogenic suspension culture of Abelmoschus esculentus L. was established. In suspension culture stage, the rule of cell growth was shown S accorded with Sigmoid Curve, the best days of sub-cultured was 4 to 8. The relationship between the dry weight of suspension cultures and pH changes in medium,the rotational speed and suspension cellulat morphology werw discussed. The effects of inoculation and AgN0₃ on the growth of embryogenic suspensions were alsodiscuss ed.The optium concentration of inoculation and level of AgN0₃ were 1.5% and 5.0 mg·L^-1,respectively.3 Regeneratated plantlets were obtained from callus differentiation. The medium of shoot induction was MS + 3.0 mg·L^-1 6-BA + 0.5 mg·L^-12,4-D+3% sucrose + 0.7% agar. The exercising seedling medium was MS+ 1.5 mg·L^-16-BA + 0.2 mg·L^-12,4-D + 1.0 mg·L^-1GA3 +3% sucrose + 0.7% agar. The medium for tube seedling root-generation was 1/2MS + 0.1 mg·L^-1 NAA. The key steps of transplanting rate is exercising seedling. The transplanting medium in rotten quality soil, vermiculite and river sand by 1 to 1 to 1 is the best treament on smelt seedling.