| This research is implemented in a factory farm which cultivates white Flammulina velutipes year-round in bags.The research material is five strains which are separated and preserved by the factory farm.The research investigates the effects of different strains in production process and compares the strains all-round screening.In the process of experiment, some original technology is further optimized.With the aid of systematic analysis of data collected during the experiment,introducing the best strain and the matching formulation of substrate,most suitable moisture and pH.The research attempts to improve the house turnover speed and provides theoretical basis of increasing benefit for enterprises by detail testing physiological maturity time and renewable time.And this research has achieved intended purpose.(1)Optimization of substrate formulation for the cultivation of white Flammulina velitupesThe extreme vertex design is used in the experiment.So we get the percentage of wheat bran,cotton seed hulls,saw-dust and corn cob,as well as find that the interaction of wheat bran and cotton seed hulls is helpful to increase the yield.The regression equation for the economical yield and substrate formulation can be got through statistical analysis.It is as follow:y=-712.845X1+321.2243X2-175.089X3-280.778X4+1954.876X1X2+2204.138X1X3+ 3680.292X1X4+506.0113X2X3+55.6585X2X4+743.3907X3X4From the regression equation an optimized formula is produced for cultivating white Flammulina velitupes:30%wheat bran,50%cotton seed hulls,10%saw-dust and 10%corn cob.(2)Optimization of moisture content in substrate for the cultivation of white Flammulina velitupesMoisture content in the substrate is critical for achieving high yield and quality.The experiment confirms that optimum moisture content after sterilization in the substrate is 60.5%-62.5%.The biology efficiency is over 90%under proper management.It is found that different strain has it's optimum substrate moisture.In the process of experiment,the variation law of the moisture loss in the cultivating bags is trace.These had not been reported in the past.(3)Optimization of pH in substrate for the cultivation of white Flammulina velitupes With the experiment done,adding 1.5%calcareousness in dry substrate could significantly improve the yield and biology efficiency,as well as shorten the cycle.The average yield of a cultivating bag is 386 gram,and biology efficiency exceeds 95%,At the same time,the cycle is significantly shortened.In the past,no one investigated the entire growth period.We did the systematic analysis for the first time.(4)Optimization the length of reactivating stage of white Flammulina velitupesThe length of reactivating has practical significance to the cyclic utilization of the mushroom house.After systematic research,it is found that:when the fructification reactivate duration is 8d,the time from opening bags to harvesting is the shortest.But the yield is the highest when the fructification regeneration duration is 10d.When the length and yield are considered comprehensively,the optimum fructification regeneration time is 8-10d for the year-round cultivation Flammulina velitupes.(5)The best cultivation time of different strainsThe optimal cultivation time is 20 days for the strain of 702,703 and 46.Their yield and biology efficiency are the highest and cycle is the shortest if cooling at this time.But for the strain of 701,biology efficiency is max when cultivating 28 days,and difference of yield is min among each bag at this time.At the same time,the efficiency of mushroom house will depress.There are different physiological maturity time and accordingly cooling time for different strains.There aren't hierarchy study before this research.(6)Comparison of different strainsThe research indicates the 704 strain is the best strain among all five strains.Its yield and biology efficiency is the highest,and the days before opening bags and cycle is shorter than others.This train is fit for production in factory farm that cultivates white Flammulina velutipes year-round.Sieving suitable strain and matching technology is the core of production in a factory farm. |