Study The Relationship Between Keratin-associated Proteins Gene (KAP And KRT) And Sheep Wool Traits

Xinjiang, an important base of sheep breeding, owing the most, is the important product-place of fine-wool and in the leading place in China.At present,domestic and international wool textile products have also to the light,soft,straight out,high-grade direction.So it is necessary to raise fine wool sheep breeding, not only for society economic but also for technology ascensive in breeding.But now, it is difficult to raise or maintain the quality of present superior fine-wool, since it is studied less about the mechanism of gene regulation, controlling the main economy traits, especially, it is dimness about the gene that have correlation with wool trait of sheep ,and we know little about they correlativity. At present the research is focus on the gene that relation with Keratin-associated proteins and Keratin intermediate filament, and the protein was construct the hair fiber. We study KAP and KRT associate gene in two way on the base of already research.Firstly, Study the genetic polymorphism of KAP and KRT gene and 5 sheep in XinjiangChoosing KAP and KRT gene (KAP1.3,KAP6-1,KAP3.2,KAP8 and KRT1.2) as candidate gene, the genetic relationship between KAP and KRT gene and different wool trait of sheep (Chinese Merino sheep, Dorset sheep, Suffolk sheep, Texel, Hu sheep) in Xinjiang were analyzed by PCR-SSCP and PCR-RFLP method. The result showed:(1) 5 different sheep was midrange polymorphism at KAP1.3 loci, and PIC was between 0.25 and 0.5. Texel sheep not achieve genetic balance tested byχ2. (2) KAP81 loci Dorset sheep was high polymorphism (PIC>0.5), Texel sheep was low polymorphism (PIC<0.25), Chinese Merino sheep, Hu sheep and Suffolk sheep was midrange polymorphism (0.25