Effects Of Spent Substrate Of Xylo-oligosaccharides From Cottonseed Hull Supplementation On Growth Metabolism Of AA Broiler Chickens

A preparation of Spent Substrate of Xylo-oligosaccharides from Cottonseed Hull(abbreviate,SSX) , which is the metabolisms of edible fungi - Pleurotus ostreatus H50, as supplementation for its effects on growth performance, slaughter parameters, metabolism of endogenous hormone, serum index and immune performance in AA broiler chickens. It is a theoretical foundation and applied reference for investing and developing functional feed additive.Chapter One The Test of Xylo-oligosaccharide Content in Spent Substrate of Cottonseed HullThe purpose of this study was to test Xylo-oligosaccharide content in Spent Substrate of Cottonseed Hull, which is applied by paper chromatography, 3,5-Dinitrosalicylic acid colorimitry(DNS) and analytical technique of photoshop. The results of statistical analysis indicated that xylobiose was the main composition of Xylo-oligosaccharides in Spent Substrate of Cottonseed Hull and xylobiose's concentration up to 55.610 mg/g.Chapter Two Effects of Spent Substrate of Xylo-oligosaccharidesfrom Cottonseed Hull supplementation on growth Metabolism of AA Broiler ChickensThe aim of this study was to investigate the effect of SSX supplementation on growth metabolism of AA broiler chickens. 288 Arbor Acress (AA) broiler chickens were chosen and divided into four groups with control group (feeding basal diets) and trial groupâ… ,â…¡andâ…¢. The experimental diets consisted of based diets supplemented with 10g, 20g and 40gSSX/Kg feed respectively which the test lasted 49 days.Results showed that: (1) Performance: Compared with control group, ADG in groupâ…¡andâ…¢increased by 3.55%(p<0.05),0.25%(p>0.05). Feed conversion ratio(FCR) in groupâ… ,â…¡andâ…¢decreased by 0.90%(p>0.05),5.18%(p<0.05),0.06%(p>0.05) respectively of 21-day-old broiler chickens. Groupâ…¡of ADG increased by 1.30%(p>0.05) and FCR decreased by 2.08%(p>0.05) of 42-day-old broiler chickens.Groupâ…¡of ADG increased by 0.19%(p>0.05) and FCR decreased by 0.77%(p>0.05) from 22 to 42 days.ADG in groupâ…¡andâ…¢increased by 1.61%(p<0.05),2.51%(p<0.05). FCR in groupâ… ,â…¡andâ…¢decreased by 3.26%(p>0.05),1.61%(p>0.05),12.25%(p<0.01) and survival rate increased by 1.45%,1.45%,4.35% of 49-day-old broiler chickens. Groupâ… andâ…¡of ADG increased by 2.21%(p>0.05),3.79%(p>0.05). Groupâ… ,â…¡andâ…¢of FCR decreased by 1.33%(p>0.05),0.95%(p>0.05),14.68%(p<0.01) from 22 to 49 days. Groupâ… andâ…¡of ADG increased by 2.21%(p>0.05),3.79%(p>0.05). Groupâ… ,â…¡andâ…¢of FCR decreased by 1.33%(p>0.05),0.95%(p>0.05),14.68%(p<0.01) from 22 to 49 days. Groupâ… ,â…¡a ndâ…¢of ADG increased by 4.42%(p>0.05),27.52%(p<0.01),30.07%(p<0.01) and of FCR decreased by 2.99%(p>0.05),23.19%(p<0.05),14.40%(p>0.05) from 43 to 49 days.(2) Slaughter parameters on 42 days: Compared with control group, Dressing percentage (DP) in groupâ… ,â…¡andâ…¢increased by 2.67%(p>0.05),3.26%(p>0.05),3.28%(p>0.05), percentage of eviscerated yield (PEY) increased by 7.74%(p>0.05),1.75%(p>0.05),6.53%(p>0.05) and percentage of half eviscerated yield (PHEY) increased by 7.98%(p<0.05),7.44%(p>0.05),8.39%(p<0.05) .Groupâ…¡of Percentage of thigh muscle yield (PTMY) increased by 3.72%(p<0.05) and groupâ… andâ…¢decreased by 6.49%(p>0.05),5.22%(p>0.05); Percentage of breast muscle yield (PBMY) in groupâ… ,â…¡a ndâ…¢decreased by 6.74%(p>0.05),2.67%(p>0.05),6.99%(p>0.05).(3) Quality of brisket: Compared with control group,Crude protein(CP) contents of brisket in groupâ…¡andâ…¢increased by 8.46%(p<0.05),6.36%(p>0.05) and groupâ… decreased by 4.54%(p>0.05) respectively. Crude fat (CF) contents of brisket in groupâ… andâ…¢decreased by 19.63%(p>0.05),33.26%(p>0.05) and groupâ…¡increased by 6.62%(p>0.05) respectively.(4) Ca,P: Compared with control group, calcium(Ca) of tibia in groupâ… ,â…¡andâ…¢ increased by (p>0.05),4.76%(p>0.05),4.52%(p>0.05) and phosphonium(P) increased by 29.64%(p<0.05),18.09%(p<0.01),48.92%(p<0.01) respectively. Ca of serum increased by 0.40%( p>0.05,groupâ… ),7.29%(p>0.05,groupâ…¢). P of serum in groupâ… ,â…¡andâ…¢increased by 15.07%(p>0.05),20.86%(p>0.05),19.23%(p>0.05).(5) Serum hormone of broilers : Compared with control group, GH in groupâ…¡andâ…¢increased by 8.06%(p<0.05) and 5.01%(p<0.05); ACTH in trial groups decreased by 3.14%,31.53%(p<0.05),28.07%(p<0.05); T3 increased by 12.89%(p<0.05),26.86%(p<0.01),22.42%(p<0.05); T4 in groupâ…¡andâ…¢increased by 22.20%(p<0.01),8.87%(p>0.05); Ins. in groupâ… andâ…¡increased by 16.04%(p<0.05),20.67%(p<0.01). Glu. in trial groups decreased by 6.43%(p>0.05),2.28%(p>0.05),10.04%(p<0.05).Testosterone of male chickens increased by 52.71%(p<0.05),17.05%(p<0.05),30.68%(p<0.05); E2 of female chickens increased by 45.29%(p>0.05),20.69%(p>0.05),51.80%(p<0.05); Follicle Stimulating Hormone(FSH) of female chickens decreased by 12.25%(p>0.05),16.23%(p>0.05).(6) Immune Function: Compared with control group, the index of thymus gland(ITG) in groupâ… ,â…¡andâ…¢increased by 29.27%(p<0.05),45.34%(p<0.01),28.96%(p<0.05) and the index of spleen(IS) increased by 2.22%(p>0.05),2.80%(p>0.05),0.93%(p>0.05) respectively. Avian Influenza Virus (AIV) H5N1 antibody titer of the trial groups increased notablely compared to the control group, which the groupâ… w as the highest. The average of AIV H5N1 antibody titer of trial groups increased by 52.70%(p<0.05),39.26%(p>0.05),37.32%(p>0.05) respectively. Newcastle Disease Virus (NDV) antibody titer of the trial groups were higher than the control group after immuned between day 11 and 35. NDV antibody titer of the groupâ…¡was up to the highest especially between day 17 and 29.But the control group step down the lowest after immuned 29 days. Compared with control group, The average NDV antibody titer increased 15.04%(p>0.05),29.75%(p>0.05),22.89%(p>0.05) respectively.