QTL Mapping Of Economic Traits On Chromosome 3 In Gushi-Anak Crossing Generation F2 Chickens

With the development of human genome research, the research of animal genomics has made huge progress. Different from human genome study, the aim of animal genomics is to look for the location chromosome, where there are quatitation trait locus(QTL).Upon this background, this thesis managed to established a chicken resource family, perform the genome scanning vis the microsatellite markers, hunt the chromosome fragments that contain the loci affecting the economical traits, and explore a proper method in the animal breeding via DNA molecular marker assistant selection as well.Gushi-Anak Crossing Generation F2 Chicks were used in the paper. Form the resulting F2. This generation hybrid chickens included 849 segregation progenies deried from seven families.10 microsatellite DNA markers on chromosome 3 were genotyped. The statistic results by Cervus2.0 software demonstrated that the allele of the selected microsatellite markers was from 2 to 5,the heterozygosity of these markers was from 0.483 to 0.706 and the polymorphism information content was from 0.367 to 0.653. These markers were a high polymorphic,which could substantially guarantee the rehabihy of the result of the research on the molecule level.The phenotypes of Experimental groups on Meat Quality Traits growth Traits, Carcass Traits Blood Biochemical Index were analyzed, The statistical analysis demonstrated there was variation for every trait in certain extent,this ensured the location of QTL.SAS statistical software in the non-balanced data analysis of variance (GLM) tags on all seats and groups carcasses traits indicators. every traits of different tag-point genotypes phenotype mean the existence of significant differences. Between 7 markers in the 10 markers were significant with meat quality Traits, 10 markers are varying degrees of related with growth Traits, Carcass Traits and Blood Biochemical Index.QTL express on line analysis program was used, the QTL mapping analysis of the every trait were carried out by interval mapping. The result indicated. 1 QTL were detected in the meat quality Traits, fat content at 103cM.20 QTL were detected in the growth Traits, 12-week-aged tibia length at 143cM; 8-week-aged chest depth at 274cM; 4-week-aged chest angle, 8-week-aged body length at 104cM; 4-week-aged body length at 253cM; 12-week-aged chest angle at 103cM; 12-week-aged body length and length of breastbo at 146cM; 4-week-aged coxa width at 243cM; we found 11 QTL infiuencing 4-week-aged shank girth ; 8-week-aged shank girth ; 4-week-aged length of breastbo; 8-week-aged length of breastbo ;0-12--week-aged weight at 255cM8 QTL were detected in the carcass Traits, wing weight at 59cM. gizzard weight at 111cM. we found 6 QTL infiuencing eviscerated yield, eviscerated yield with giblet, pancreas weight, leg muscle weight and breast muscle weight and carcass weight at 255cM5 QTL were detected in the blood biochemical index, GGT at 162cM. AKP at 67cM. CHE at 59cM. HDL at 216cM. CK at 142cM.