| In order to selected the anti-heat stress traditional chinese medicine compounds on dairy cows,In this study, on the basis of clearing away summer heat and heat evil, dissipating dampness, calm the nerves, regulating vital energy, promoting digestion, the three different chinese herbal medicine prescriptions were organized with elsholtzia splendens,baikal skullcap root, Ophiopogon japonicus, mint, Akabia Stem, Rice Paper Plant Pith, Wrinkled Gianthyssop Herb, Capillary Wormwood Herb, Tatarinow Sweetflag Rhizome, honeysuckle, Spine Date Seed, hawthorn, Magnolia officinalis,Citrus,licorice, etc, Manufactured by artificial heat stress model in mice,and different Chinese Traditional Medicine decoction were given to the mice, compared with heat stress-related clinical symptomsphysiol and ogical and biochemical indexes between the treatment group and the control group, Selected the ideal prevention and treatment prescriptions on heat stress, By its application to test dairy cows, the results Proved that, to a certain extent, can prevent and mitigate on dairy cows under heat stress.The experimental results of screening Prescription showed that: All the three Prescription could improve the clinical symptomsphysiol and physiological and biochemical indexes of blood in heat stress mice. Among them, the middle dose Play the best effect :under the heat stress, the K+,Cl-,AST,ALT concentration in positive control group were increased significantly than those in negative control group(P<0.01), but Na+,TP, ALB,AKP concentration in positive control group were decreased significantly than those in negative control group(P<0.01).The middle dose of"Kangshuyihao"could improve the physiological and biochemical indexes of blood in heat stress mice, compared with the mice in positive control group, the effects were significant(P<0.01). Na+, K+,TP,ALT concentration could restore to the normal level.In order to study the effect and mechanism of"anti-summerâ… "to heat stress, the subject selected five weight, age, parity, milk yield similar to the health of Holstein cows to test, the clinical trial results showed that:under the heat stress, the average respiratory rate and rectal temperature of dairy cows were significantly higher than room temperature phase (P <0.01), But the average daily milk yield, milk fat, milk protein rate was significantly lower (P <0.01).Cows after treatment, the average respiratory rate and rectal temperature were higher than the average stage of heat stress are decreased, but the difference was not significant (P> 0.05),while the milk yield,milk fat,milk protein were significantly recovered (P <0.01).Under the heat stress,the dairy cows sera Na+ concentration decreased than the negative control group (P> 0.05), and the K+ and Cl- concentration increased than the negative control group, Among them, K+ difference was not significant (P > 0.05), Cl--significant difference (P <0.01).After usage of traditional Chinese medicine prescription"Kangshu I", Na+ concentration recovered than the positive control group (P> 0.05). K+ concentration decreased than the positive control group (P> 0.05); Cl- concentration was significantly lower than the positive control group (P <0.01).Under the heat stress, dairy cow sera TP,G concentration decreased than the negative control group (P<0.05); ALB concentration decreased than the negative control group (P> 0.05). After usage of traditional Chinese medicine prescription"Kangshu I",TP,G concentrations showed a rising trend than the positive group, and compare with the negative control group, was significant difference (P<0.05); ALB concentration showed a rising trend than the positive control group, but compare with the negative control group, the difference was not significant (P>0.05).Under the heat stress,the dairy cows sera ALT,AST concentration in positive control group showed an upward trend than the negative control group,ALT was not significant (P> 0.05), AST was significantly different (P <0.01);sera AKP concentration was decreas (P> 0.05). After usage of traditional Chinese medicine prescription"Kangshu I", the dairy cows sera ALT,AST concentration in positive control group showed a decreasing trend,ALT was not significant (P> 0.05),AST was significantly different (P <0.01);serum AKP concentration showed an upward trend (P> 0.05).Under the heat stress,the dairy cows sera HSP70 concentration in positive control group increased significantly than those in negative control group(P<0.01);The plasma ALD concentration, sera COR and T4 concentrations decreased significantly than those in negative control group (P <0.01); ACTH decreased significantly than those in negative control group (P<0.05), T3 decreased non-significantly than those in negative control group (P>0.05). After usage of traditional Chinese medicine prescription"Kangshu I", HSP70 levels in drug treatment group increased non-significantly than those in the positive control group (P>0.05). The plasma ALD concentrations,sera COR and T4 concentrations were significantly higher than those in the positive control group (P<0.01). ACTH increased significantly than those in the positive control group (P <0.05), T3 increased non-significantly than those in the positive control group (P> 0.05). That can only indicated the Chinese Medicines could prevent and alleviate the heat stress in cows at a certain extent.
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