The Expression Of Stress Activated Protein Kinase,Fas And FasL In Benign Prostate Hyperplasia,Prostatic Introepithelial Neoplasia And Prostatic Cancer

Objective Cancer of the prostate is the most prevalent malignancy and the second-highest fatal neoplasm among Western males. Recently, it has been shown that the incidence rate of the disease increased from year to year in China. Thus, the study of the mechanism of prostatic carcinogenesis and tumour development is of increasing interest. JNK, > Fas and FasL expressions were examined by immunohistochemistry in BPH> PIN and Pea, and their relationship to apoptosis in this tissues was determined. Our destination is to determine the effect of expression of INK, in Fas/FasL induced apoptosis. Another aim of the study is to address the mechanism of PIN and Pea resist to Fas/FasL induced apoptosis so that we can find a key therapeutic target inhibiting the growth of prostate tumors.Methods Expression of INK, > Fas and FasL were examined byimmunohistochemistry in 10 cases of BPH > 10 cases of PIN and 10 cases of Pca.The number of apoptotic cells per thousand (%o) was determined by counting, under high power (X 400), the cell identified on the H & E section as apoptotic. A minimum of 2000 cells were evaluated for each section and up to 5 sections per patient. Statistical methods: rank sum test and rank correlation test. Comparisons are not significant unless the corresponding P-value is less than 0.05.Results Immune reactivity for JNK-! antiboby was present in the cytoplasm of both secretory and basal cells of BPH; nuclear staining was seen in approximately 45% of the secretory cells. Immune reactivity for INK] antibody was present in the cytoplasm and membrane of PIN lesions. Occasional nuclear staining was detected in high-grade PIN. Nuclear staining was seen in approximately 1% in low-grade PIN. INK, protein expression was present in the cytoplasm of Pea; nuclear staining was hardly ever seen. The expression of JNK, in BPI^ PIN and Pea was increased in turn.(P<0.05). Fas and FasL expression was detected in all samples of BPH ^ PIN and Pea tissue. Fas expression is mainly as membranous type in BPH and PIN. The difference of Fas expression between BPH and PIN was no significant(P<0.05). Fas expression in Pea was cytoplasmic-membranous type and significant higher than that in BPH and PIN (P<0.05). FasL expression is mainly as cytoplasmic type in BPH and as membranous type in PIN. FasL expression in Pea was cytoplasmic-membranous type. From BPH> PIN to Pea, FasL expression was significant high in turns (P<0.05). The apoptotic index in BPH, PIN and Pea was 1.79%, 6.43 %o and 11.81%o. The expressions of INK, > Fas and FasL were correlation with the percent apoptosis in BPFL PIN and Pea.Conclusions The expression of INK > Fas and FasL were detected in all samples of BPH > PIN and Pea tissues and there was a tendency to be increased. Phosphorylated and activated INK translocates into the nuclens where it can phosphorylate a number of transcription factors. In PIN and Pea there were less nuclear staining, which can not induce apoptosis. Persistence of INK activation plays a key role in Fas/FasL mediated apoptosis. For the activation of INK maintained in PIN and Pea is transient, the latters are insensitive to Fas/FasL mediated apoptosis. The expression of FasL in both PIN and Pea tissue plays a key role for tummours to escape immune surveillance. This results may provide us with clues for a better understanding of prostate cancerprogression, and for the development of new strategies for treatment of Pea by regulate the activation of JNk.