The Studies On Pharmacologic Effect Of TAOSHUGEN Extract On Rat Nonbacterial Prostatitis And Its Anti-inflammation Mechanisms

To study the effects of TAOSHUGEN(TSG) extract on rat nonbacterial prostatitis and its anti-inflammation mechanisms. Methods: (1)The experimental rat prostatitis made by injecting carrageenan into the prostatic gland, Croton oil-induced ear swelling , carrageenan-induced hind paw edema, the increase in celiac vascular permeability caused by acetic acid, the mouse pleurisy induced by carrageenan, and the weight of cotton granuloma in mice were prepared to study the effects of TSG extract on inflammation. (2)The residual contents of both Congo Red and carbon mote in mice blood were measured by the ultraviolet spectrophotometry to investigate the effect of TSG on nonpeculiar immune function. Its effect on mouse spleenlymphocyte proliferation induced by ConA was tested by MTT assay. (3)The bacteriostasis was conducted by the testube and plat methods. Qualitative analysis of TSG extract was also tested. Results: The weight -s of rat prostate in groups treated with TSG extract were significantly lighter (P<0.05) than that in the model group. The total population of WBC in rat prostate fluid was decreased (p<0.05), and the density of lecithin corpuscle was increased (p<0.05) , and the imflammatory cell infiltration and fiber hyperplasia of prostate were also relieved compared with the model control observed with optical microscope. The ear edema induced by Croton oil, paw edema induced by carrageenan, the increase in vascular permeability caused by acetic acid and the weight of cotton granuloma in mice were inhibited significantly by TSG extract (P<0.05). It inhibited the chemotaxis of WBC, but not significant statistical difference(p>0.05). TSG inhibited the synthesis of PGE2 and formation of MDA (P<0.05), and elevated the activity of SOD (P<0.05) in abdominal exudates caused by acetic acid. TSG extract strengthened the function of engulfing Congo Red and enhanced the index of phagocytosis of carbon mote in mice(P<0.05). It also stimulated the proliferation of mouse spleen lymphocyte induced by ConA. TSG extract significantly inhibited the growth of Bacillus coli, Bacillus proteus, and staphylococcus aureus, and the MIC was 0.002,0.032,0.016g·ml-1, respectively. Thedeterminations of flavons, anthraquinones, phenols and tannin, phytosterols, triterpenoids were positive findings. Conclusion: TSG extract has a significantly therapeutic effect on rat experimental nonbacterial prostatitis caused by carrageenan. Its anti-inflammation mechanism is possible to be related to strengthening the phagocytosis of macrophages, promoting T lymphocyte proliferation and decreasing PGE2 level.