| Objective: To observe the curative effects of Decoction Shenkangling, having the function of "Benefiting the kidney and Promoting blood ciuculation", on the adriamycin-induced nephrotic (AN) rats, and meanwhile to research the relationship among nuclear factor-Kappa B(NF- K B), thromboxane A2 (TXA2) , prostacyclin (PGI2) and other factors in AN rats, then to explain the pathogenesis of nephrotic syndrome (NS) and the mechanisms of Chinese medication.Methods: Using adriamycin with the dosage of 5.5mg/kg to inject the caudal vein of 50 rats to produce the animal models similar to mankind minimal change nephropathy (MCD). Then these rats were averagely divided randomly into 4 groups, namely Group B (Pathologic Contrast Group) and the other three Treated Groups including Group C (Prednisone), Group D (Prednisone plus Decoction Shenkangling), Group E (Prednisone plus Decoction Zhibodihuang) and Group F (Prednisone plus Decoction Taohongsiwu). Synchronously, Normal Contrast Group (Group A) with 10 rats was established. Before the treatment and after 3-week's treatment, NF-K B, TXB2 and 6-keto-PGF1 a of plasm and urine, urine protein of 24 hours, as well as serum albumin, cholesterol and triglyceride of all rats were tested. And their renal pathomorphology was also observed by light microscope and electron microscope. Statistical analysis was performed by SPSS 11.0 system software.Results: Compared with Group A, in Group B, its general status was significantly worse; its urine protein increased clearly, albumin decreased markedly, cholesterol, triglyceride, TXB2 of plasm and urine and the ratio of TXB2/6-keto-PGF1 a increased distinctly; simultaneously, the activity of NF-K.B enhanced evidently, p<0.01 all; Moreover, its renal pathomorphology aggravated. After treatment, compared with Group B, the general status of alltreated rats recovered in different level. In Group C, all the above-mentioned indexes ameliorated obviously (p<0.05) other than TXB2 and 6-keto-PGF] a (p>0.05). However, in Group D, Group E and Group F, all the above-mentioned indexes ameliorated much more obviously (p<0.01). In Group D and Group F, the renal lesion markedly lessened; while in Group C and Group E, it didn't present. Compared with Group C, in Group D, all the above-mentioned indexes improved distinctly (p<0.01 or p<0.05); in Group E, its cholesterol reduced observably (p<0.05); and in Group F, its plasm 6-keto-PGF| a gained markedly (p<0.05). The difference in increasing albumin between Group D and Group E was significant (p<0.05), but it was much more obviously between Group D and Group F (p<0.01). Besides, the significance in elevating plasm 6-keto-PGF1 a between Group D and Group E was great (p<0.05). Last, there was no statistical significance of other indexes among Group D, Group E and Group F (p>0.05).Conclusions:1. Animal model similar to mankind nephrotic syndrome can be achieved by injecting adriamycin into the caudal vein of rat once with the dosage of 5.5mg/kg, and its clinical situation resembles the Symptom of "Kidney Deficiency and Blood Stasis" in TCM.2.The clinical situation and progression of AN rats may be related to their further increase of the activity of NF- K B, the disequilibrium of the ratio of TXA2/PGI2 and Neuro-endocrine-immune (NEI) Network, which led to the action of inflammatory-sclerotic media and the hypercoagulable state. Besides, the further increase of urine protein, cholesterol, triglyceride and further decrease of albumin and the processing lesion of kidney (including the damage of renal tubule and stroma) may also be concerned with their episode. Among the above-mentioned, the increase of the activity of NF- K. B was the key point.3.The clinical situation of the disequilibrium of NEI Network is similar to the Symptom of "Kidney Deficiency and Blood Stasis" in TCM. Decoction Shenkangling with the function of "Benefiting the kidney and Promoting blood circulation" can remarkably ameliorate the clinical situation and experimental indexes of the Symptom of "Kidney Deficiency and B...
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