Experimental And Clinical Study On Treatment Of Stress Urinary Incontinence With The Mixture Of Suo Quan Gu Niao

Objective:To assess the security and effectiveness of the mixture of Suo Quan Gu Niao(SQGN) through trial of acute toxicity,effects of contractile activity of bladder smooth muscle and sphincter urethrae in vitro and clinical research. In order to develop the new traditional Chinese medicine to treat Stress Urinary Incontinence on the base of this study .Methods:Trial of acute toxicity.The mice were given once the mixture of SQGN 300% by oral irrigation in the pre-trival.If the number of dead mice exceeds 70%, 50% lethal dose(LD50) was measured ;when LD50 could not be measured because of lower toxicity,the maximum tolerance dose(MTD) was measured with the highest concentration 300% and the largest volume 0.3ml/10g by irrigating gastric(ig) for 3 times in one day. Trial in vitro:The bladder muscle strip prepare along the dorsal iongitudinal axis in rabbit bladder body and sphincter urethrae were suspended in a tissue chamber, their isometric contractile activity signals were measured by the multichannel recorder through musde-tension transducer; 54 pre-clinical cases were proceed in order to observe the clinical effects of the mixture of suo quan gu niao.Results: The pre-trials showed that the LD50 could not be measured in the trials of acute toxicity , and the MTD was 180g/Kg after the revision of its measurment which was corresponding to 108 times of daily clinical dosage. Trial in vitro made clear that it could remarkably increase the contractile of sphincter urethrae , inhibite the urinary bladder smooth muscle contraction and lower the baseline; when the mixture of Suo Quan Gu Niao was 25mg /ml(high dosage), it could inhibit the urinary bladder strip contraction induced by KCL,too.Pre-clinical made clear that it could remarkably improve the smptoms of patients who were diagnosed as weakness of spleen and kidney.The examination of urine dynamics showed that the MFR,AMFR and S were remarkably improved, FUL was prolonged, MCUP was incrcascd;but their was not remarkable difference between the bladder dctrusor, and the negative stress wave did not appear;and the serum estradiol(E2) lever was increased.The overall effect was better than the control group and did not has obvious poisonous effect and side-effect.Conclusion:The Mixture of SQGN has little poisonous effect and side-effect. The MTD measured has important significance to research the dosage of long toxicity trial, and we could research the safe and effective dosage in clinic,avoid acute side-effect according to it.It could remarkably increase the contractilation of Sphincter Urethrae of rabbits in vitro and inhibite contractile of urinary blader strip.It has effect of invigorating the kidney and spleen, supplementing the vital energy and pycnosising. It could improve paraments of urine dynamics, and increase the serum E2 level. Its overall effect is better than the contorl group. The treatment mechanism probably is relative to increase the serum E2 lever, and excite the a epinephrine receptor.