| Hemorragic shock is an important cause of traumatic death in emergency medicine.It expresses mainly the inadequate microcirculatory perfusion and metabolic dysfunction of cells,and results in the ischemia-hypoxia injury of multiple tissue and organs,exen multiple system organ failure(MSOF).The mechanism is still not very clear,but it is generally accepted that oxygen free radicals,[Ca2+]i overloading and energy metabolism disorders play an important role in the damages of tissue and organs.It has been shown that frustose-1,6-diphosphate(FDP) is a significant intracellular metabolite which plays a direct role in regulating the energy metabolism and the activity of enzyme.So FDP can supply the energy to the major tissue and organs in hemorragic shock,and stabilize the cell membrane,and inhibit the generation of oxygen free radicals.It is reported that magnesium is the natural calcium-channel blocking and regulates all enzymatic reactions. Moreover,magnesium can reduce the damage of oxygen free radicals and improve the microcirculatory.Therefore working on the premise,it is speculated that sodium magnesium fructose diphosphate (FDPM) which is in part substituted magnesium iron for sodium in fructose-1,6-diphosphate can take a synergetic effect to protect the tissue and organs of hemorragic shock.This experiment mainly study the benefical effects of FDPM on hemorragic shock in rats.Is FDPM more effective therapy on hemorragic shock than FDP?The mechanism of protection is unknow. It remains a matter for further investigation.Objective:To study the effective therapy of FDPM on the hemorragic shock rats, and observe survival rate and time, and explore the protection of multiple organs(heart,liver and kidney). Methods:One hundred twenty Wistar rats were used and divided into six groups randomly:normal saline control group,large dose of FDPM treated group,middle dose of FDPM treated group,small dose of FDPM treated group,FDP treated group and magnesium sulfate treated group.The present study has been carried using Wigger,s hemorragic shock model.At the different time,the changes of mean arterial pressure(MAP) were monitored,and the survival rate and time of hemorragic shock rats were observed.Moreover,the specimens of heart,liver,kindey and serum were determined.Results:(1) The therapy effects of FDPM improved signifcantly the MAP of hemorragic shock rats and increased survival rate and time of rats.The effects of FDPM was mostly superior to control groups.These results indicate that FDPM can treat markedly the hemorragic shock.(2)FDPM lowered the activity of creatine phosphokinase(CK) and lactic dehydrogenase(LDH) in serum,and improved the activity of Na+-K+- ATPase,Ca2+-ATPase,Mg2+-ATPase and superoxide dismutase(SOD) in myocardium,and reduced the content of lipid peroxidation (malondiadehyde,MDA)in myocardium.The results indicate that FDPM can restore and enhance energy metabolism and improve the activity of ATPase,and attenuate tissue injury caused by free radicals,and decrease [Ca2+]i overloading in myocardium.(3)FDPM reduced the activity of alanine transaminase(ALT) and aspartate transaminase(AST) in serum,and increased the activity of Na+-K+-ATPase,Ca2+-ATPase,Mg2+-ATPase and superoxide dismutase(SOD) in liver,and decreased the content of lipid peroxidation (malondiadehyde,MDA) in liver. The results indicate that FDPM can restore and enhance energy metabolism and improve the activity of ATPase,and attenuate tissue injury caused by free radicals,and decrease [Ca2+]i overloading in liver.(4)FDPM reduced the content of blood urea nitrogen(BUN) and creatinine(Cr),and increased the activity of Na+-K+-ATPase,Ca2+-ATPase,Mg2+-ATPase and superoxide dismutase(SOD)in kindey,and decreased the content of lipid peroxidation (malondiadehyde,MDA)in kindey. The results indicate that FDPM can restore and enhance energy metabolism and improve the activity of ATPase,and attenuate tissue injury caused by free radicals,and decrease [Ca2+]i o...
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