Experimental Study Of The Deep Fascia Microcirculation After Acute High Tension Electrical Burns Of Rabbits

Objective: The study was designed to investigate the microcirculation changes and rules of the periphery tissue by watching the deep fascia microcirculation of the healthy limb ends after acute high tension electrical burn, and at the same time to explore the microcirculation changes of the whole body.Methods: First, the twenty-eight adult healthy rabbits, regardless of sex, female rabbits of which were not pregnant , were randomly divided into two equal number groups: One is high tension electrical burn group and another is control group. Second, the rabbits were prepared by cleaning fur at the day before experiment, and at the same time the experimental rabbits were forbidden to eating food 8h before experiment. Third, the right hind limbs of the rabbits of the experimental group were subjected to 10000V,77 ~ 95mA charge of alternative electric current for 5 seconds, which was the method of duplicating high tension electrical burn model; and then the windows for researching the deep fascia microcirculation of the left hind limb ends were made by the new method of "Opening window with dropping. As far as the control group was concerned, there was no change except thehigh tension electrical burn factor. The two groups were made as that mentioned above in turn. After that, the blood vessel factors such as shape, distinctness, caliber, and blood vessel density, the factors around vessel such as exudation, hemorrhage, and the factors in the vessel such as blood flow velocity, erythrocyte aggregation, and white microembolus of the arteriole, blood capillary, postcapillary venule and venule were measured at time intervals of before electrical burn, at once,0.5h,2h,4h,6h after electrical burn (such was to say To> Ti > T2> T^^ T4^ T5 phase)by WX-9B model multipart microscope and the computer picture processing system. Fifth, to velocity of blood flow, the method of Tian Niu's was absorbed; and the distinctness of the micro vascular as well as the degree of the erythrocyte aggregation was divided into three levels; and the white microembolus was measured by the number of white microembolus in the blood capillary; and the caliber was directly measured by computer picture processing system; and the density of the blood vessel was measured by the method of crux intermultiplication; and the shape of the blood vessel was described by three independent cases. Sixth, to the statistics of blood flow velocity and microvascular distinctness , the method of Wilcoxon rank sum test was used between the correlated phases of the two groups; to the statistics of the erythrocyte aggregation, microvascular exudation and hemorrhage, the method of Fisher exact test was used between the correlated phases of the two groups; to the statistics of the microvascular density and caliber, the method ofpaired T test was used between To phase and the others in the two groups; to the statistics of the microvascular density and caliber, the method of unitization comparative T test was used between the correlated phases of the two groups; in general, to the statistics of the microvascular density and caliber, the method of one factor repeated measures data statistical analysis was used between the two groups. The statistics mentioned above was dealt with in the SAS system (V 6.12).As far as the white microembolus was concerned, it was directly described.Results: After high voltage electrical burn, it was seen that the blood vessel density was less(P<0.01),and then to the normal level after 30 minutes; the arteriole was contracted(P< 0.01),then to the normal level 30 minutes late; and the distinctness of arteriole became poor 6h after electrical burn(P <0.01); and there was no change in the shape; furthermore, there was no change around and in the vessel. To the blood capillary, the caliber value was less(P<0.01),and then to the normal level after 30 minutes; the distinctness was poorer after 4h(P<0.01); the white microembolus was fund at once after electrical burn by 3 of the 14 rabbits, and to its highest point at 4h fast by 193, and then the level of the white microembolus became low soon by 95; however, there was no change like that above of the control group: five white microemboluses were found at 30min after electrical burn; one were found at 2h; nine at 4h; eight at 6h . There were no remarkable change about the erythrocyte aggregation and the blood flow velocity. As far as...