Exprimental Study On Effects Of Octreotide To Hepatic Artery Embolization On The Therapy Of Rat Liver Cancer

Objective To observe if octreotide can resist angiogenesis induced byhepatic artery embolization and augment the inhibiting effect on tumor.Methods Walker256 carcinoma cells were transplanted into rat's liverto create liver cancer model. Rats bearing tumor were randomly dividedinto four groups.A-control group,B-octreotide group,C-HAE group,D-HAEplus octreotide group. Change of tumor volume and tumor inhibiting rateafter therapy was evaluated.The expression of VEGF ,TIMP1and MVD in tumortissue was detected by immunohistochemical.Results The tumor volume in group B,C,D was smaller than that in groupA(P<0.01),and the tumor volume in group D was smaller than that in groupC too(P<0.05).The expression of VEGF in group C increased significantlycompared with group A(P<0.05), and that in group B decreased compared withgroup A, but the difference had no significance(P>0.05). However, theexpression of VEGF in group D decreased significantly compared with groupC(P<0.05). The expression of TIMP1 decreased slightly but notsignificantly in group C compared with that in group A(P>0.05). That ingroup B increased significantly compared with group A(P<0.05), and thatin group D also increased significantly compared with group C(P<0.05).The expression of MVD increased slightly but not significantly in groupC compared with that in group A(P>0.05). But that in group B decreasedsignificantly compared with group A(P<0.05), and that in group D alsodecreased significantly compared with group C(P<0.05).Conclusion Octreotide plus hepatic arterial embolization can decreasethe high expression of VEGF in tumor tissue by only hepatic arteryembolization and increased the expression of TIMP in tumor tissue, reducedthe angiogenesis of tumor and futher augment the inhibiting effect ontumor in the therapy of rat liver cancer.