Influence Of QiLeng Decoction On Expression Of Nestin Protein After Focal Cerebral Ischemic Reperfusion In Rats

Objective:To explore the influence of QiLeng decoction on expression of Nestin protein after focal cerebral ischemic reperfusion in rats ,and to provide the scientific foundation for benefit vital energy (BVE), nourishing yin(NY),activating blood circulation(ABC) on treating ischemic stroke. Methods:The rats were randomly divided into four groups including sham-operation group,model group, QiLengQuYangYingYao(QLQYYY) decoction group and QiLeng decoction group. The model of focal cerebral ischemia reperfusion injury were was made by using thread embolizing and fastening of the ICA in rats. Neurological grade were evaluated. Nestin protein were observed by immunohistochemical method at different time point after cerebral ischemia reperfusion. The cerebral cell morpathology was examined by HE. Results:(1) The cerebral structure in sham-operation group was normal, while that in the modle group showed ischemic.Contrasting with model grou, QLQYYY decoction group and QiLeng decoction group could improve the ischemic pathological changes of brain in rats much better at the end of 24th hour after reperfusion ,and the effect of QiLeng decoction group is superior to that of QLQYYY decoction group. (2) The neurography grade of sham-operation group were normal. There was statistic significance among model group, QLQYYY decoction group and QiLeng decoction group in evaluation of neurography grade(P<0.05). The effect of QiLeng decoction group is superior to that of other group.(3)The expression of Nestin protein was not found in sham-operation group. There was statistic significance among model group, QLQYYY decoction group and QiLeng decoction group in expression of Nestin protein (P<0.05),and The efect of QiLeng decoction group is superior to that of other group. Conclusion:QiLeng decoction play the neuron protective effects after cerebral ischemic...