The Experiment Of The Effect Of Carthamus Tinctorius And Angelica Sinensis Injection On The Limb Ischemia-reperfusion

Objective: To investigate the protective effects of Carthamustinctorius and Angelica sinensis injection on the limb ischemia-reperfusion.Methods: 56 healthy male S-D rats were randomly divides intocontrol group, ischemia-reperfusion group and medicine-intervenedgroup(which was injected Carthamus tinctorius and Angelica sinensisinjection). Ischemia-reperfusion group and medicine-intervened groupwere divided into 2 hours, 4hours and 8hours group respectivelyaccording to the different reperfusion time points. After the establishmentof the limb ischemia-reperfusion rat model, each group was observed bymeasuring the concentration of superoxide dismutase(SOD),malondialdehyde(MDA),myeloperoxidase (MPO) in the blood serumand skeletal muscle,and the concentration of tumor necrosis factor-alpha(TNF-a) and nitrogen monoxidum(NO) in the blood serum, theextedema of the skeletal muscle, and the expression of Bcl-2 and BAXprotein in skeletal muscle.Histological examination of the skeletal musclewas studied under a light.Result: The concentration of SOD in the blood serum and skeletalmuscle in the ischemia-reperfusion group and in the medicine-intervenedgroup was junior to it in the control group, Statistical analysis(one-wayANOVA) shows the level of the ischemia-reperfusion was lower than the control group(P＜0.05) and the level of the medicine intervention goupwas lower than the ischemia reperfusion group at the different time pointafter reperfusion(P＜0.05).The concentration of MDA,MPO in the bloodserum and skeletal muscle in the ischemia-reperfusion group, theconcentration of TNF-a,NO in the blood serum in theischernia-reperfusion group were superior to that in the control group,Statistical analysis shows that there is significant difference in the level ofthose between the ischemia-reperfusion group and the controlgroup(P＜0.05). The concentration of MDA,MPO in the blood serum andskeletal muscle in the medicine-intervened group, and the concentrationof TNF-a,NO in the blood serum in the medicine-intervened group werelower than that in the ischemia-reperfusion group, and Statistical analysisshows the differences between the medicine-intervened group andischemia-reperfusion group are significant(P＜0.05). Skeletal musclestriations disappearance, blood vessel hyperaemia, inflammation wereobserved in the ischemia- reperfusion group by light microscope, andeven skeletal muscle cell disruption and degeneration was observed in the8 hours group in the ischernia-reperfusion group, all were not observed inthe control group, and the change was weakened in the medicine group.The increasing expression of BAX protein was observed in theischemia-reperfusion group as the time go on, the expression of Bcl-2protein increased at the beginning, and then declined. Statistical analysis shows that there was no statisical difference between the 2h group ofischemia -reperfusion group and medicine-intervened group(P＞0.05), butthere were significant statisical differences in the 4h group and 8h groupof ischemia-reperfusion group and medicine-intervened group(P＜0.05).Conclusion: After reperfusion, the concentration of SOD in theblood serum and skeletal muscle declined, while the concentration ofMDA, MPO in the blood serum and skeletal muscle and the concentrationof TNF-a,NO in the blood serum increased, which can bring damage tothe limb skeletal muscle. Carthamus tinctorius and Angelica sinensisinjection can weaken the damage after limb ischemia-reperfusion.