Expression And Correlations Of Survivin,bcl-2 And PTEN In The Human Astrocytomas

Objective: To observe the expression and correlations of survivin,bcl-2 and PTEN in the human astrocytomas,investigate their effect on the occurrence,development and the clinical pathological parameter of human astrocytomas,and set up the foundation of early diagnosis,malignant degree diagnosis and prognostic evaluation of human astrocytomas.Methods: 67 cases of human astrocytomas (grade I 16 cases,grade II 17 cases,grade III 17 cases,grade IV 17 cases) for experiment group and 10 cases of normal brain tissue for control group. Applying EIivision immunohistochemical technique to perform examination and SPSS statistical software to calculate the value ofχ~2 and Spearman's correlation coefficient.Result: The positive percentage of survivin,bcl-2 and PTEN was 47.76%,44.78%,52.24% in 67 cases of human astrocytomas respectively. The expression of survivin,bcl-2 was negative in 10 cases of normal brain tissue,but PTEN was positive in them. Fisher's Exact Test:there was significant difference of survivin,bcl-2 and PTEN expression among the different grades.There was significant difference of the survivin,bcl-2 and PTEN expression between lower grade (I～II) and higher grade (III～IV)(P<0.01),the value ofχ~2 was 15.575,11.236,11.678 respectively. Along with the pathological grade increasing of human astrocytomas , the positive percentage and expressive intensity of survivin,bcl-2 increased gradually,but PTEN decreased gradually.There was positive correlation between the expression of survivin and bcl-2 in human astrocytomas,but negative correlation was found between the expression of survivin and PTEN,bcl-2 and PTEN.Conclusions: 1. The high expression of survivin and bcl-2 and the relative low expression of PTEN correlated with the occurrence and development of human astrocytomas; 2.The expression of survivin,bcl-2 and PTEN correlated with the pathological grade and malignant degree; 3.The reactivation of survivin and bcl-2 and mutation or loss of PTEN aren't isolated events. There might be some cooperation among them; 4.The contribution of survivin,bcl-2 and PTEN in human astrocytomas offer some theoretical informations for the research of clinical neotype molecule target pharmacal therapy.