The Concentration Of Neuron-specific Enolase And S-100β Protein In Blood Serum And Cerebrospinal Fluid Of Patients With Herpes Simplex Encephalitis And Its Clinical Significance

Objective To investigate the changes of Neuron-specific enolase(NSE)content and S-100βprotein in serum and cerebrospinal fluid from patients with Herpes simplex encephalitis and to find out the correlation with anomaly electro-encephalogram and Cranial MRIMethods Twenty-one cases with Herpes simplex encephalitis were collected as observe group.At the same period,10 patients were admitted as the control group who had headache and indication of lumbar puncture and were diagnosed as neurosis or migraine lastly.NSE and S-100βprotein of cerebrospinal fluid and serum was detected by enzyme-linked immunosorbent assay of din-antibody with filling Group was compared with the single-factor analysis of variance.The groups were compared using paired t-test information and Pearson(Pearson) correlation analysis methods were compared.P＜0.05 was implicated for the difference between statistical significance.Abnormal EEG is classified into mild,moderate and severe abnormalities.The numbers were 6 cases,7 cases,4 cases.The 21 cases were divided into 17 abnormal cases and 4 normal cases.The Patients were respectively measured with NSE and S-100βprotein of their blood and cerebrospinal fluid.All experimental data were using mean±standard deviation(X±S). Comparison analysis was performed by Spss 10.0 statistical package.Results Patients with acute Herpes simplex encephalitis has a significantly higher content of NSE and S-100βprotein of blood and cerebrospinal fluid than that of recovery group and control group(P＜0.01);Content of NSE and S-100βprotein of cerebrospinal fluid is significantly higher than that of blood in patients with light,midrange and weight anomaly electroencephalogram(P＜0.05);Content of NSE and S-100βprotein of cerebrospinal fluid and blood in patents with midrange and weight anomaly electroencephalogram is higher than that in patient with light anomaly electroencephalogram(P＜0.05).NSE levels of acute blood were significantly associated with that of cerebrospinal fluid(r= 0.792,P＜0.01);S-100βprotein levels of acute blood were significantly associated with that of cerebrospinal fluid(r=0.847,P＜0.01).NSE and S-100βcontent in blood and cerebrospinal fluid of 17 cases with abnormal MRI and 4 cases is higher than that of 10 cases of the control group(p＜0.01);17 cases with abnormal MRI was no significant difference from 4 cases with normal MRI(P＞0.05).Conclusion NSE and S-100βprotein in serum and cerebrospinal fluid of healthy people are rarely.When infected with herpes simplex virus,menstruation cycle of the virus penetrated the blood-brain barrier breakthroughs in the central nervous system.NSE and S-100βprotein in serum and cerebrospinal fluid cannot diagnostic herpes simplex virus encephalitis specificity.The content of NSE of blood and cerebrospinal fluid in patients with acute viral encephalitis indicates the severity of the disease of the extent of lesion of neuralgia cell.It is a useful biochemical maker of brain-tissue damage.