Analysis On The Pathological Characteristic Of Murine Cervicitis Caused By Ureaplasma Urealyticum Serotype 3 Or Chlamydia Trachomatis Serotype D And Mixed Infection

ObjectiveTo analyse the pathological characteristic of murine cervicitis infected by the only ureaplasma urealyticum(UU) or the only chlamydia trachomatis(CT) and the two mixed microorganism.In the purpose of masterying more knowledges about the pathogenicity of the two kind of microorganism.MethodFrom 56 female mices,48 were selected in random to be inoculated with UU serotpe 3 with concentration moer than 10~6copy/g just in the day of treatment with estradiol. After 7 days,secretion from those murine canals were collected for UU culture. And we got 32 UU positive mices.The UU positive mices were divided into two groups ,that's group A and B.There were 16 mices in each group.Mices in group A were further inoculated with CT serotype D from vagino after grouping.In group B, mices were not inoculated with CT serotpe D.And the rest 8 mices that were never inoculated with UU were built up group C.Those mices in group C were only inoculated with CT serotype D after 7 days of treatment with estradiol.21 days later all the mices were killed and cervical swabs were taken so that we had detection by both culture and FQ-PCR and had an analysis on pathogenic microorganism.The tissue of mice cervical canals were retented for pathological research and were detected by both scanning electron microscope(SEM) and transmission electron microscope(TEM).Result(1) There is little difference of behavior modification among all the mices. (2) We successfully find some cervicitis animal models infected by UU+CT.(3) We find that 9 mices are infected with both UU and CT in group A,and 7 mices are infected only by UU in group B,and 4 mices are infected only by CT in group C.(4) Non-specific inflammation in sole UU infected cervicitis and cervicitis represented typical follicular cervicitis in sole CT infection and pantomorphic cervicitis in mixed infection.(5) Under SEM baculiform UU was found on the mucous membrane epithelial cell of cervical canas in the case of sole UU infection, and no microorganism was found but structure badly harmed in the case of sole CT infection, and in the mixed infection there were many spherical CT and filamentous UU hidden in the seriously damaged cilia and microvilli on the superficies of epithelial cell.(6) Under TEM in sole UU infective cervicitis, epithelial cell alined in order and cilia broke off, and segmented neutrophile granulocytes were found in desmohemoblast;in sole CT infective cervicitis, reticular body(RB) of CT was found in the cytoplasma of macrophagocyte and more segmented neutrophile granulocytes infiltrated desmohemoblast;in the mixed infective cervicitis, epithelia cell was more seriously damaged and the most inflammatory cell whose nucleus exhibited polymorphism and segmented infiltrated desmohemoblast.Each and every periods of CT, including RB,metaphase and maturation phase were found in macrophagocyte which also was full of lysosome.But there wasn't any change of ultrastructure in the control group.ConclusionThere are differents in the characteristic of murine cervicitis caused by sole UU or CT and the mixed microorganism infected. Moreover the pathological damnification is much severe in the mixed infected mices.