P504S/HMWCK/P63 Triple Cocktail Stain In Adenocarcinoma Of The Prostate

Objective: A cocktail of three antibodies, P504S, HMWCK and p63, was applied to the prostate specimens immunohistochemically, and investigated the utilization of the antibody for diagnosing prostatic intraepithelial neoplasia (PIN), and atypical adenomatous hyperplasia (AAH) from true cancer.Materials and methods: 44 cases of prostate cancer including 39 cases from radical prostatectomy and 5 cases of transurethral resection of prostate (TUR-P), 5 cases of benign prosthetic hyperplasia were reviewed. PIN was observed the point of HGPIN and LGPIN. For to lesion P504S/HMWCK/ p63 were applied using a double-chromogen stain to discriminate the cancer from non-cancer lesions.Results: Of these 44 cases, 37 (84%) cases were diffuse strongly positive, and 7cases (16%) were focally negative for P504S staining. HMWCK/p63 usually used for demonstrating basal cells were negative in the cases of prostatetic adenocarcinoma with Gleason grade 2 and 3, but interestingly 11% adenocarcinoma with Gleason grade 4 and 5 showed focally or scattered positive. High grade intraepithelial nepplasia (HGPIN) represented moderate to strong positive for P504S, while low grade intraepithelial neoplasia ( LGPIN ) were negative to weak positive. In adjacent to HGPIN, well differentiated adenocarcinoma were often found, and partly showing a sequential change from strong to weak in the same gland. The majority of AAH (5/6 cases) was negative for P504S, but positive for HMWCK/p63. in benign prostatic glands, 21 percent of total cases were faintly positive for P504S and all cases were presented strongly for HMWCK/p63.Conclusion: Triple antibodies allowed us to easily discriminate the benign atypical lesion from well differentiated adennocarcinoma with Gleason grade 2 and 3 pattern, which has been difficult by usual HE stain and by each antibody separately. It is revealed that HGPIN is proved to be a premalignant lesion strongly associated with carcinoma occurrence by immunohistochemical data as well as histological features. AAH is possibly belongs benigen lesion.