The Mechanism Of Affecting Ulcer Healing Of Butylamine Roxatidine Acetate In Gastric Ulcer Rats Indued By Acetic Acid

The chemical formula of BRA is C18H29N3O2,which is a new Roxatidine derivate(The chemical formula is C17H26N2O3) from the revised and modified Acetid Roxatiding. It is a new H2-recepton imtagonist that first combined by us in the world and reported by CA. The related researches on pharmcological action and it is system has not been reported at home on abroad. The research of the project may fill the blank of how BRA defense gastric ulcer both in and outside China ,and provide valuable research proof for exploiting new and effective H2-receptor antagonist. The mechanism of the experiment haven't been reported.Objective:Test based on the model of A-rat, searching the influnce of BRA to the recovery, the content of NO in the A-rat's serum and gastric tissue, and the expression of TGF-β1 protein, discussing the mechanism on the ulcer, provide the basis in developing H2-receptor antagonist.Method:1.Divide 48 Wistar rat into 6 groups, 8 for each group.(1)Blank control group, do intragastric administration with physiological saline.(2)Ulcer control group, do intragastric administration with physiological saline.(3)⁵ BRA in three different grades of dose. (28mg/kg,14mg/kg,7mg/kg )(6) Known control group with ranitidine.(27mg/kg)Prepare Acetic acid burned in gastric rat(called A-rat) model, except the Blank control group.Start to drug the A-rat intragastrically once for each 15 days after the model was made for 3 days. Fasting after the last taking of drug (drinking water free). Give cervical death on the 16th day. Measure the dose of NO in the serum and gastric tissue of A-rat though Biochemical method. Immune tissue chemical coloration to observe TGF-β1 protein expression in mucosa of gastric.Result:1.BRA promotes the recovery of the ulcer rat,similar to ranitidine.2.The dose measurement of NO in the rat's serum and gastric tissue shows,large and small close of BRA both promote the SOD in its serum and gastric tissue,P<0.01-0.05 compared with the control group.3. Immune tissue chemical coloration shows the weak possitive expression of TGF-β1 on the edge of the ulcer of the blank group, weaker than the modle group. BRA in high and medium dose and ranitidine control group the expression of TGF-β1 increase obviously. BRA in low dose the expression of TGF-β1 do not increase obviously. The result shows, during the treatment, the dose depending promote the expression of TGF-β1, attach to the recovery of the ulcer.Conclusion:BRA reduces the area of gastric ulcer and the gastric mucosa injury of A-rats, promoting recovery, increases the content of NO in the A-rat's serum and gastric tissue, promotes the expression of TGF-β1 protein, enhances the ability of tissue repairing, functions in preventing gastric ulcer actively.