An Experimental Study Of Tuo Min Zu Fang In Antagonizing Hypersensitivity Type Ⅰ

Objective To study the effects and pharmacological mechanisms of Tuo Min Zu Fang (TMZF) in treating experimental hypersensitivity typeⅠin vitro.Methods 1.The effects of Tuo Min Zu Fang to ileum excited by histamine.We connected the ileum of guinea pig to instrument.Experiments were composed by four parts.①Movement of ileum excited by histamine②The treatment group of TMZF in two concentrations:1.4g/ml,2.8g/ml and the treatment group of of Loratadine(0.5 mg/ml).We recorded the ileum's movement after adding different drugs.We evaluated the effects of Tuo Min Zu Fang according to the numerical values of ileum's contraction.H ematoxylin and eosin were used to stain the cell models to observe the morphologic changes after treated with TMZE 2. The effects of Tuo Min Zu Fang to ileum excited by Compound48/80.Experiments were composed by four parts.①Movement of ileum excited by Compound48/80②The treatment group of TMZF in two concentrations:1.4g/ml,2.8g/ml and the treatment group of of Ketotifen(0.4 mg/ml).We recorded the ileum's movement after adding different drugs.We evaluated the effects of Tuo Min Zu Fang according to the numerical values of ileum's contraction.H ematoxylin and eosin and were used to stain the cell models to observe the morphologic changes after treated with TMZEResults TMZF 1.4g/ml and 2.8g/ml significantly inhibit the ileum's movement excited by histamine(t=2.194,3.325,P＜0.05) and the ileum's movement excited by Compound48/80 (t=2.314,3.455,P＜0.05).Conclusions It is suggested that TMZF possesses an antagonistic effect on hypersensitivity typeⅠ.TMZF may stabilize mast cell membrane and inhibit the degranulation of mast cells,and this is one of the main mechanisms of TMZF's effects of anti-hypersensitivity typeⅠ.