Effects Of Astragalus Polysaccharide On Maturation Of Exosomes Derived From Dendritic Cells Of Leukemia Patients

Objective Exosomes are a kind of bioactive vescules serected by a multitude of many cell types.The study is that dendritic cell derived-exosome(DEX) is pulsed tumor antigens and can induce a strong immune attack on an individual's cancer if redelivered in sufficient volume. This experiment is to explore the agumentation of Astragalus Polysaccharide(APS) on surface molecular expression of exosomes in vitro derived from dentritic cells by bone marrow mononuclear cells (BMNCs).Methods The BMNCs from acute mylocytic leukemia complete remission (AML-CR) patients were cultured in RPMI1640 medium containing Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor (rHuGM-CSF) /Recombinant Human interleukin 4(rHuIL-4) placed in 5%CO₂ in air at 37℃incubator.After 7 days,positive control group is affected by a conventional dose of Lipopolysaccharide(LPS),the experimental groups(low,adequate,high dose APS groups) were stimulated by different doses of APS,negative control group was treated by RPMI1640 medium of an equal volume.After 36h,exosomes were extracted from the supernatant of DCs by repeated ultracentrefugation.The morphologic features of exosomes were observed by transmission electron microscope(TEM) and the exosomal phenotype was detected by flow cytometry(FCM);besides,the dentritic cells were identified by Wright's staining and FCM.Results There was no statistical significance about exosomal morphology among different groups. Compared with control groups,adequate APS groups increased the expression of HLA-DR,CD80,CD86 on the DEX and had a statistical significance(P<0.05).Conclusion The adequate APS could enhance the monocular expression on DEX in AML-CR patients;besides,the expression content is related with the different doses.